ZFIN ID: ZDB-PUB-101108-25
Investigating regeneration and functional integration of CNS neurons: Lessons from zebrafish genetics and other fish species
Fleisch, V.C., Fraser, B., and Allison, W.T.
Date: 2011
Source: Biochimica et biophysica acta. Molecular basis of disease   1812(3): 364-380 (Review)
Registered Authors: Allison, Ted, Fleisch, Valerie
Keywords: Zebrafish, Regeneration, Retina, Optic nerve, Spinal cord, Brain, Central nervous system, Stem cell, Glia
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation
  • Central Nervous System/physiology*
  • Fishes/embryology
  • Fishes/genetics*
  • Nerve Regeneration/physiology*
  • Neurons/cytology
  • Neurons/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed: 21044883 Full text @ BBA Molecular Basis of Disease
Zebrafish possess a robust, innate CNS regenerative ability. Combined with their genetic tractability and vertebrate CNS architecture, this ability makes zebrafish an attractive model to gain requisite knowledge for clinical CNS regeneration. In treatment of neurological disorders, one can envisage replacing lost neurons through stem cell therapy or through activation of latent stem cells in the CNS. Here we review the evidence that radial glia are a major source of CNS stem cells in zebrafish and thus activation of radial glia is an attractive therapeutic target. We discuss the regenerative potential and the molecular mechanisms thereof, in the zebrafish spinal cord, retina, optic nerve and higher brain centres. We evaluate various cell ablation paradigms developed to induce regeneration, with particular emphasis on the need for (high throughput) indicators that neuronal regeneration has restored sensory or motor function. We also examine the potential confound that regeneration imposes as the community develops zebrafish models of neurodegeneration. We conclude that zebrafish combine several characters that make them a potent resource for testing hypotheses and discovering therapeutic targets in functional CNS regeneration.