PUBLICATION
Characterization of spatial and temporal expression pattern of Col15a1b during zebrafish development
- Authors
- Bretaud, S., Pagnon-Minot, A., Guillon, E., Ruggiero, F., and Le Guellec, D.
- ID
- ZDB-PUB-101108-24
- Date
- 2011
- Source
- Gene expression patterns : GEP 11(1-2): 129-134 (Journal)
- Registered Authors
- Le Guellec, Dominique
- Keywords
- Zebrafish, Teleost, Danio rerio, In situ hybridization, Expression pattern, Extracellular matrix, Multiplexins, Collagen XV, Muscle, Adaxial cells, Otic vesicle, Eye, Telencephalon, Hindbrain, Aortic arches
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Collagen/genetics*
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental*
- In Situ Hybridization
- Molecular Sequence Data
- Sequence Alignment
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- PubMed
- 21044700 Full text @ Gene Expr. Patterns
Citation
Bretaud, S., Pagnon-Minot, A., Guillon, E., Ruggiero, F., and Le Guellec, D. (2011) Characterization of spatial and temporal expression pattern of Col15a1b during zebrafish development. Gene expression patterns : GEP. 11(1-2):129-134.
Abstract
In mammals, collagen XV is primarily expressed in skeletal and cardiac muscles, and loss of its expression in mice results in a mild skeletal myopathy. We recently identified Col15a1a, a zebrafish ortholog of the human collagen XV gene which expression was restricted to notochord in embryos. Col15a1a knockdown led to defects in muscle maintenance via Shh signaling. Here we report that zebrafish express a second ortholog Col15a1b. The identification of its complete primary sequence showed that the overall structure of collagen XV is well conserved between vertebrates. Whole mount in situ hybridization and RT-PCR analysis revealed that at 12 hpf Col15a1b is mainly expressed in slow muscle cell lineage and in nervous tissues, and, at later stages transcripts are detected in eyes, otic placodes and aortic arches. Based on the expression pattern of col15a1b, sequence alignments and synteny comparisons, we conclude that, contrary to collagen XVa, the zebrafish collagen XVb likely displays the same or similar function than the mamalian orthologs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping