PUBLICATION
Cypermethrin has the potential to induce hepatic oxidative stress, DNA damage and apoptosis in adult zebrafish (Danio rerio)
- Authors
- Jin, Y., Zheng, S., Pu, Y., Shu, L., Sun, L., Liu, W., and Fu, Z.
- ID
- ZDB-PUB-101027-28
- Date
- 2011
- Source
- Chemosphere 82(3): 398-404 (Journal)
- Registered Authors
- Keywords
- Cypermethrin, Zebrafish, Oxidative stress, DNA damage, Apoptosis, Gene transcription
- MeSH Terms
-
- Animals
- Apoptosis/drug effects
- DNA Damage
- Female
- Gene Expression/drug effects
- Insecticides/toxicity*
- Liver/drug effects*
- Liver/metabolism
- Liver/pathology
- Mutagens/toxicity*
- Oxidative Stress/drug effects
- Pyrethrins/toxicity*
- RNA, Messenger/metabolism
- Transcription, Genetic/drug effects
- Zebrafish*
- Zebrafish Proteins/metabolism
- PubMed
- 20965546 Full text @ Chemosphere
- CTD
- 20965546
Citation
Jin, Y., Zheng, S., Pu, Y., Shu, L., Sun, L., Liu, W., and Fu, Z. (2011) Cypermethrin has the potential to induce hepatic oxidative stress, DNA damage and apoptosis in adult zebrafish (Danio rerio). Chemosphere. 82(3):398-404.
Abstract
Cypermethrin (CYP), a widely used Type II pyrethroid pesticide, is one of the most common contaminants in the freshwater aquatic system. We studied the effects of CYP exposure on the induction of hepatic oxidative stress, DNA damage and the alteration of gene expression related to apoptosis in adult zebrafish. Hepatic mRNA levels for the genes encoding antioxidant proteins, such as Cu/Zn-Sod, Mn-Sod, Cat, and Gpx, were significantly upregulated when zebrafish were exposed to various concentrations of CYP for 4 or 8days. In addition, the main genes related to fatty acid β-oxidation and the mitochondrial genes related to respiration and ATP synthesis were also significantly upregulated after exposure to high concentrations (1 and 3μgL(-1)) of CYP for 4 or 8days. Moreover, in a comet assay of zebrafish hepatocytes, tail DNA, tail length, tail moment and Olive tail moment increased in a concentration-dependent manner. The significant induction (p<0.01) of all four parameters observed with CYP concentrations of 0.3μgL(-1) or higher suggests that heavy DNA damage was induced even at low levels. Furthermore, several apoptosis- related genes, such as p53, Apaf1 and Cas3, were significantly upregulated after CYP exposure, and Bcl2/Bax expression ratio decreased, especially in groups treated with 1 and 3μgL(-1) CYP for 8days. Taken together, our results suggested that CYP has the potential to induce hepatic oxidative stress, DNA damage and apoptosis in zebrafish. This information will be helpful in fully understanding the mechanism of aquatic toxicology induced by CYP in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping