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ZFIN ID: ZDB-PUB-101013-1
Molecular and expression analysis of apolipoprotein E gene in the Chinese sturgeon, Acipenser sinensis
Li, C.J., Gan, F., Chen, X.H., Liu, Z.G., Li, L.X., Wei, Q.W., and Tang, Y.K.
Date: 2011
Source: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 158(1): 64-70 (Journal)
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Keywords: Chinese sturgeon, Apolipoprotein E, Cloning, RT-PCR, gene expression
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Apolipoproteins E/chemistry*
  • Apolipoproteins E/genetics*
  • Cloning, Molecular
  • Fishes*/genetics
  • Gene Expression Profiling
  • Molecular Sequence Data
  • Phylogeny
  • Protein Conformation
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Species Specificity
PubMed: 20868764 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
ABSTRACT
Apolipoproteins are carrier proteins that bind to lipids to form lipoprotein particles and have been shown to play an important role in lipid metabolism. In this study, a full-length cDNA for apolipoprotein E, named AsapoE, was cloned from the Chinese sturgeon (Acipenser sinensis). This cDNA sequence is 1289bp in length, and codes for a polypeptide of 274 amino acid residues, which is 45% and 42% identical to that of the rainbow trout and zebrafish, respectively, and 39%, 30%, and 29% identical to frog, mouse, and human respectively. The predicted AsApoE protein has a conserved amphipathic α-helix region with the potential to bind to lipids. RT-PCR analysis reveals that AsapoE is expressed in all tissues examined with a preferential expression in the kidney and liver. During the embryo development stage, AsapoE mRNA is low but still detectable at gastrula stage embryos; then AsapoE mRNAs reach a higher level in muscle contraction stage embryos, this relatively stable expression persists during the following embryogenic stages and declines 1day after hatching. These results will serve as a basis for comparative studies on vertebrate apoE genes.
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