PUBLICATION
An Essential and Evolutionarily Conserved Role of Protein Arginine Methyltransferase 1 for Adult Intestinal Stem Cells During Postembryonic Development
- Authors
- Matsuda, H., and Shi, Y.B.
- ID
- ZDB-PUB-101011-8
- Date
- 2010
- Source
- Stem cells (Dayton, Ohio) 28(11): 2073-2083 (Journal)
- Registered Authors
- Matsuda, Hiroki
- Keywords
- adult organ-specific stem cell, histone arginine methyltransferase, transcriptional coactivator, thyroid hormone receptor, dedifferentiation
- MeSH Terms
-
- Adult Stem Cells/cytology*
- Adult Stem Cells/metabolism*
- Animals
- Cell Proliferation
- Epithelial Cells/cytology
- Epithelial Cells/metabolism
- Gene Expression Regulation, Developmental/genetics
- Gene Expression Regulation, Developmental/physiology
- Immunohistochemistry
- In Situ Hybridization
- Intestines/cytology*
- Mice
- Protein-Arginine N-Methyltransferases/genetics
- Protein-Arginine N-Methyltransferases/metabolism*
- Xenopus laevis
- PubMed
- 20872846 Full text @ Stem Cells
Citation
Matsuda, H., and Shi, Y.B. (2010) An Essential and Evolutionarily Conserved Role of Protein Arginine Methyltransferase 1 for Adult Intestinal Stem Cells During Postembryonic Development. Stem cells (Dayton, Ohio). 28(11):2073-2083.
Abstract
Organ-specific adult stem cells are critical for the homeostasis of adult organs and organ repair and regeneration. Unfortunately, it has been difficult to investigate the origins of these stem cells and the mechanisms of their development, especially in mammals. Intestinal remodeling during frog metamorphosis offers a unique opportunity for such studies. During the transition from an herbivorous tadpole to a carnivorous frog, the intestine is completely remodeled as the larval epithelial cells undergo apoptotic degeneration and are replaced by adult epithelial cells developed de novo. The entire metamorphic process is under the control of thyroid hormone, making it possible to control the development of the adult intestinal stem cells. We show here that the thyroid hormone receptor-coactivator PRMT1 (protein arginine methyltransferase 1) is upregulated in a small number of larval epithelial cells and that these cells dedifferentiate to become the adult stem cells. More importantly, transgenic overexpression of PRMT1 leads to increased adult stem cells in the intestine and conversely knocking down the expression of endogenous PRMT1 reduces the adult stem cell population. In addition, PRMT1 expression pattern during zebrafish and mouse development suggests that PRMT1 may play an evolutionally conserved role in the development of adult intestinal stem cells throughout vertebrates. These findings are not only important for the understanding of organ-specific adult stem cell development but also have important implications in regenerative medicine of the digestive tract.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping