PUBLICATION

Sox17 and chordin are required for formation of Kupffer's vesicle and left-right asymmetry determination in zebrafish

Authors
Aamar, E., and Dawid, I.B.
ID
ZDB-PUB-101011-54
Date
2010
Source
Developmental dynamics : an official publication of the American Association of Anatomists   239(11): 2980-2988 (Journal)
Registered Authors
Aamar, Emil, Dawid, Igor B.
Keywords
Sox17, Chordin, Kupffer's vesicle (KV), left-right asymmetry, dorsal forerunner cells (DFC)
MeSH Terms
  • Animals
  • Body Fluids/metabolism*
  • Body Patterning/genetics
  • Body Patterning/physiology*
  • Embryo, Nonmammalian/cytology*
  • Embryo, Nonmammalian/metabolism*
  • Gene Expression Regulation, Developmental/genetics
  • Gene Expression Regulation, Developmental/physiology
  • Glycoproteins/genetics
  • Glycoproteins/metabolism*
  • Immunohistochemistry
  • In Situ Hybridization
  • Intercellular Signaling Peptides and Proteins/genetics
  • Intercellular Signaling Peptides and Proteins/metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • SOXF Transcription Factors/genetics
  • SOXF Transcription Factors/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
20925124 Full text @ Dev. Dyn.
Abstract
Kupffer's vesicle (KV), a ciliated fluid-filled sphere in the zebrafish embryo with a critical role in laterality determination, is derived from a group of superficial cells in the organizer region of the gastrula named the dorsal forerunner cells (DFC). We have examined the role of the expression of sox17 and chordin (chd) in the DFC in KV formation and laterality determination. Whereas sox17 was known to be expressed in DFC, its function in these cells was not studied before. Further, expression of chd in these cells has not been reported previously. Targeted knockdown of Sox17 and Chd in DFC led to aberrant Left-Right (L-R) asymmetry establishment, as visualized by the expression of southpaw and lefty, and heart and pancreas placement in the embryo. These defects correlated with the formation of small KVs with apparently diminished cilia, consistent with the known requirement for ciliary function in the laterality organ for the establishment of L-R asymmetry.
Genes / Markers
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Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes