PUBLICATION
Expression of paired-like homeodomain transcription factor 2c (PITX2c) in epidermal keratinocytes
- Authors
- Shi, G., Sohn, K.C., Choi, T.Y., Choi, D.K., Lee, S.S., Ou, B.S., Kim, S., Lee, Y.H., Yoon, T.J., Kim, S.J., Lee, Y., Seo, Y.J., Lee, J.H., and Kim, C.D.
- ID
- ZDB-PUB-101011-13
- Date
- 2010
- Source
- Experimental cell research 316(19): 3263-3271 (Journal)
- Registered Authors
- Choi, Tae-Young, Kim, Chang Deok
- Keywords
- Paired-like homeodomain transcription factor 2, Keratinocytes, Proliferation, Differentiation, Zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation
- Cell Proliferation
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Epidermis/cytology*
- Homeodomain Proteins/metabolism*
- Humans
- Keratinocytes/cytology
- Keratinocytes/metabolism*
- Transcription Factors/metabolism*
- Zebrafish/embryology
- PubMed
- 20875405 Full text @ Exp. Cell Res.
Citation
Shi, G., Sohn, K.C., Choi, T.Y., Choi, D.K., Lee, S.S., Ou, B.S., Kim, S., Lee, Y.H., Yoon, T.J., Kim, S.J., Lee, Y., Seo, Y.J., Lee, J.H., and Kim, C.D. (2010) Expression of paired-like homeodomain transcription factor 2c (PITX2c) in epidermal keratinocytes. Experimental cell research. 316(19):3263-3271.
Abstract
Paired-like homeodomain transcription factor 2 (PITX2) has been implicated as one of the genes responsible for Rieger syndrome. It has been also shown to play a central role during development. In this study, we investigated the functional role of PITX2 in keratinocyte differentiation. RT-PCR analysis showed that PITX2c isoform was predominantly expressed in a differentiation-dependent manner. Consistent with, immunohistochemical staining showed that PITX2 expression was increased in the upper layer of epidermis. When PITX2c was overexpressed in cultured keratinocytes by a recombinant adenovirus, the differentiation markers such as involucrin and loricrin were significantly increased at both mRNA and protein levels. In addition, PITX2c overexpression led to the decrease of cell growth, concomitantly with the upregulation of cell cycle-related genes p21. To investigate the effect of PITX2c in vivo, we microinjected PITX2c expression vector into zebrafish embryo. Interestingly, overexpression of PITX2c in zebrafish embryo led to the formation of horn-like structure and thickening of epidermis, together with the increase of keratin 8 (K8) expression. These results suggest that PITX2c has a role in proliferation and differentiation of epidermal keratinocytes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping