PUBLICATION

Characterization of Mesonephric Development and Regeneration Using Transgenic Zebrafish

Authors
Zhou, W., Boucher, R.C., Bollig, F., Englert, C., and Hildebrandt, F.
ID
ZDB-PUB-100910-21
Date
2010
Source
American journal of physiology. Renal physiology   299(5): F1040-F1047 (Journal)
Registered Authors
Bollig, Frank, Englert, Christoph, Zhou, Weibin
Keywords
wt1, nephron, gentamicin, renal injury
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cadherins/genetics
  • Cloning, Molecular
  • Genes, Wilms Tumor/physiology
  • Genetic Markers
  • Gentamicins
  • Green Fluorescent Proteins
  • Image Processing, Computer-Assisted
  • Immunohistochemistry
  • Intracellular Signaling Peptides and Proteins/genetics
  • Kidney/cytology
  • Kidney/growth & development
  • Kidney/physiology
  • Membrane Proteins/genetics
  • Nephrons/growth & development*
  • Nephrons/physiology*
  • Plasmids/genetics
  • Protein Synthesis Inhibitors
  • Regeneration/physiology*
  • Renal Insufficiency/chemically induced
  • Renal Insufficiency/pathology
  • Stem Cells/physiology
  • Zebrafish/physiology*
  • Zebrafish Proteins/genetics
PubMed
20810610 Full text @ Am. J. Physiol. Renal Physiol.
Abstract
The zebrafish is a valuable vertebrate model for kidney research. The majority of previous studies have focused on the pronephros of zebrafish, which consists of only two nephrons and is structurally simpler than the mesonephros of adult fish and the metanephros of mammals. To evaluate the zebrafish system for more complex studies of kidney development and regeneration, we investigated the development and post-injury regeneration of the mesonephros in adult zebrafish. Utilizing two transgenic zebrafish lines (wt1b::GFP and pod::NTR-mCherry), we characterized the developmental stages of individual mesonephric nephrons and the temporal-spatial pattern of mesonephrogenesis. We found that mesonephrogenesis continues throughout the life of zebrafish, with a rapid growth phase during the juvenile period and a slower phase in adulthood such that the total nephron number of juvenile and adult fish linearly correlates with body mass. Following gentamicin-induced renal injury, the zebrafish mesonephros can undergo de novo regeneration of mesonephric nephrons, a process known as neo-nephrogenesis. We found that wt1b expression was induced in individually dispersed cells in the mesonephric interstitia as early as 48 hours following injury. These wt1b-expressing cells formed aggregates by 72-96 hours following injury which proceeded to form nephrons. This suggests that wt1b may serve as an early marker of fated renal progenitor cells. The synchronous nature of regenerative neo-nephrogenesis suggests that this process may be useful for studies of nephron development.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes