PUBLICATION
Characterization of Mesonephric Development and Regeneration Using Transgenic Zebrafish
- Authors
- Zhou, W., Boucher, R.C., Bollig, F., Englert, C., and Hildebrandt, F.
- ID
- ZDB-PUB-100910-21
- Date
- 2010
- Source
- American journal of physiology. Renal physiology 299(5): F1040-F1047 (Journal)
- Registered Authors
- Bollig, Frank, Englert, Christoph, Hildebrandt, Friedhelm, Zhou, Weibin
- Keywords
- wt1, nephron, gentamicin, renal injury
- MeSH Terms
-
- Cloning, Molecular
- Nephrons/growth & development*
- Nephrons/physiology*
- Regeneration/physiology*
- Zebrafish/physiology*
- Renal Insufficiency/chemically induced
- Renal Insufficiency/pathology
- Kidney/cytology
- Kidney/growth & development
- Kidney/physiology
- Cadherins/genetics
- Stem Cells/physiology
- Protein Synthesis Inhibitors
- Zebrafish Proteins/genetics
- Gentamicins
- Membrane Proteins/genetics
- Animals, Genetically Modified
- Genes, Wilms Tumor/physiology
- Image Processing, Computer-Assisted
- Genetic Markers
- Immunohistochemistry
- Animals
- Plasmids/genetics
- Green Fluorescent Proteins
- Intracellular Signaling Peptides and Proteins/genetics
- PubMed
- 20810610 Full text @ Am. J. Physiol. Renal Physiol.
Citation
Zhou, W., Boucher, R.C., Bollig, F., Englert, C., and Hildebrandt, F. (2010) Characterization of Mesonephric Development and Regeneration Using Transgenic Zebrafish. American journal of physiology. Renal physiology. 299(5):F1040-F1047.
Abstract
The zebrafish is a valuable vertebrate model for kidney research. The majority of previous studies have focused on the pronephros of zebrafish, which consists of only two nephrons and is structurally simpler than the mesonephros of adult fish and the metanephros of mammals. To evaluate the zebrafish system for more complex studies of kidney development and regeneration, we investigated the development and post-injury regeneration of the mesonephros in adult zebrafish. Utilizing two transgenic zebrafish lines (wt1b::GFP and pod::NTR-mCherry), we characterized the developmental stages of individual mesonephric nephrons and the temporal-spatial pattern of mesonephrogenesis. We found that mesonephrogenesis continues throughout the life of zebrafish, with a rapid growth phase during the juvenile period and a slower phase in adulthood such that the total nephron number of juvenile and adult fish linearly correlates with body mass. Following gentamicin-induced renal injury, the zebrafish mesonephros can undergo de novo regeneration of mesonephric nephrons, a process known as neo-nephrogenesis. We found that wt1b expression was induced in individually dispersed cells in the mesonephric interstitia as early as 48 hours following injury. These wt1b-expressing cells formed aggregates by 72-96 hours following injury which proceeded to form nephrons. This suggests that wt1b may serve as an early marker of fated renal progenitor cells. The synchronous nature of regenerative neo-nephrogenesis suggests that this process may be useful for studies of nephron development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping