PUBLICATION

Imaging Cilia in Zebrafish

Authors
Jaffe, K.M., Thiberge, S.Y., Bisher, M.E., and Burdine, R.D.
ID
ZDB-PUB-100820-30
Date
2010
Source
Methods in cell biology   97C: 415-435 (Chapter)
Registered Authors
Burdine, Rebecca, Jaffe, Kimberly
Keywords
none
MeSH Terms
  • Animals
  • Cilia/chemistry
  • Cilia/metabolism
  • Cilia/physiology
  • Cilia/ultrastructure*
  • Cryoultramicrotomy/methods
  • Embryo, Nonmammalian
  • Humans
  • Microscopy/methods*
  • Models, Biological
  • Movement/physiology
  • Zebrafish*/embryology
  • Zebrafish*/physiology
PubMed
20719283 Full text @ Meth. Cell. Biol.
Abstract
Research focused on cilia as extremely important cellular organelles has flourished in recent years. A thorough understanding of cilia regulation and function is critical, as disruptions of cilia structure and/or function have been linked to numerous human diseases and disorders. The tropical freshwater zebrafish is an excellent model organism in which to study cilia structure and function. We can readily image cilia and their motility in embryonic structures including Kupffer's vesicle during somite stages and the pronephros from 1 day postfertilization onward. Here, we describe how to image cilia by whole-mount immunofluorescence, transverse cryosection/immunohistochemistry, and transmission electron microscopy. We also describe how to obtain videos of cilia motility in living embryos.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping