ZFIN ID: ZDB-PUB-100820-12
Dual oxidase in the intestinal epithelium of zebrafish larvae has anti-bacterial properties
Flores, M.V., Crawford, K.C., Pullin, L.M., Hall, C.J., Crosier, K.E., and Crosier, P.S.
Date: 2010
Source: Biochemical and Biophysical Research Communications   400(1): 164-168 (Journal)
Registered Authors: Crawford, Katie, Crosier, Kathy, Crosier, Phil, Flores, Maria, Hall, Chris, Pullin, Lisa
Keywords: Dual oxidase, reactive oxygen species, intestine, zebrafish, inflammatory bowel disease, innate immunity
MeSH Terms:
  • Animals
  • Gene Knockdown Techniques
  • Intestinal Mucosa/enzymology*
  • Larva/enzymology
  • NADPH Oxidases/classification
  • NADPH Oxidases/genetics
  • NADPH Oxidases/physiology*
  • Phylogeny
  • Reactive Oxygen Species/metabolism
  • Salmonella typhimurium
  • Zebrafish/metabolism*
  • Zebrafish/microbiology
PubMed: 20709024 Full text @ Biochem. Biophys. Res. Commun.
Reactive oxygen species (ROS) function in a range of physiological processes such as growth, metabolism and signalling, and also have a pathological role. Recent research highlighted the requirement for ROS generated by Dual oxidase (DUOX) in host-defence responses in innate immunity and inflammatory disorders such as inflammatory bowel disease (IBD), but in vivo evidence to support this has, to date, been lacking. In order to investigate the involvement of Duox in gut immunity, we characterized the zebrafish ortholog of the human DUOX genes. Zebrafish duox is highly expressed in intestinal epithelial cells. Knockdown of Duox impaired larval capacity to control enteric Salmonella infection.