PUBLICATION
Zebrafish Nanos interacts with and regulates the phosphorylation of Mylz2
- Authors
- Xu, Y., Wang, H., Zhou, J., Lei, Y., Zhou, Y., Yang, Q., Ye, D., Li, W., and Deng, F.
- ID
- ZDB-PUB-100806-4
- Date
- 2010
- Source
- Biochimie 92(12): 1812-1817 (Journal)
- Registered Authors
- Ye, Ding
- Keywords
- Nanos, Mylz2, Interaction, Phosphorylation
- MeSH Terms
-
- Protein Binding
- RNA-Binding Proteins
- Myosin Light Chains/genetics
- Myosin Light Chains/metabolism*
- Mutation
- HEK293 Cells
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Glutathione Transferase/genetics
- Glutathione Transferase/metabolism
- Phosphorylation
- Cardiac Myosins/genetics
- Cardiac Myosins/metabolism*
- Protein Interaction Mapping/methods
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Gene Library
- Immunoprecipitation
- Animals
- Binding Sites/genetics
- Humans
- Blotting, Western
- Two-Hybrid System Techniques
- PubMed
- 20673786 Full text @ Biochimie
Citation
Xu, Y., Wang, H., Zhou, J., Lei, Y., Zhou, Y., Yang, Q., Ye, D., Li, W., and Deng, F. (2010) Zebrafish Nanos interacts with and regulates the phosphorylation of Mylz2. Biochimie. 92(12):1812-1817.
Abstract
Nanos genes were required for differentiation of the anterior-posterior body axis in the Drosophila embryo. In addition to this somatic function, the nanos protein plays critical roles in the migration and survival of PGCs in vertebrates and invertebrates, but the regulating mechanisms of Nanos is largely undefined during the embyro development.In this study, we report a novel interaction between zebrafish Nanos and myosin light chain II (Mylz2). This stable complex, which is formed through the RNA-binding zinc finger domain and fourteen amino acids at the C-terminus of Nanos, was confirmed by GST pull-down and co-immunoprecipitation. Both of these two parts are required for the interaction with Mylz2, but nity-one amio acids at N-terminus of Nanos are not required. Using a phospho-myosin light chain 2 (Ser19) antibody, we demonstrate that Nanos downregulates the phosphorylation of the Mylz2 by cotransfecting PRK-FLAG-Na and PRK-HA-My Mylz2 in 93T cell. We discuss the biological roles of the interaction between Nanos and Mylz2 and the Nanos regulating phosphorylation of the Mylz2 in PGCs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping