Small Molecule Screening in Zebrafish
- Peal, D.S., Peterson, R.T., and Milan, D.
- Journal of cardiovascular translational research 3(5): 454-460 (Review)
- Registered Authors
- Milan, David J., Peterson, Randall
- Chemical Biology, Zebrafish, Small Molecule Screen
- MeSH Terms
- Biological Assay*
- Disease Models, Animal
- Drug Discovery/methods*
- Gene Expression Regulation, Developmental/drug effects
- Molecular Targeted Therapy*
- Signal Transduction/drug effects
- Small Molecule Libraries*
- Translational Research, Biomedical*
- 20680709 Full text @ J.Cardiovasc. Transl. Res.
Peal, D.S., Peterson, R.T., and Milan, D. (2010) Small Molecule Screening in Zebrafish. Journal of cardiovascular translational research. 3(5):454-460.
Small molecule screens have become useful tools in ongoing efforts to understand complex biologic systems and disease states. These screens can identify compounds that specifically affect signaling pathways, development, and disease processes. The zebrafish Danio rerio has increasingly been used as a whole organism model in which to perform such functional small molecule screens. Here, we review recent advances in screening approaches that focus on modulation of developmental processes and signaling pathways, along with suppressor screens of disease models. The identification of small molecule targets continues to be a challenge, and several recent approaches to this problem are discussed. The promise of chemical screens in zebrafish to identify novel biologic probes and therapeutic compounds continues to drive this rapidly growing field.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes