PUBLICATION

The in vivo performance of plasmonic nanobubbles as cell theranostic agents in zebrafish hosting prostate cancer xenografts

Authors
Wagner, D.S., Delk, N.A., Lukianova-Hleb, E.Y., Hafner, J.H., Farach-Carson, M.C., and Lapotko, D.O.
ID
ZDB-PUB-100719-58
Date
2010
Source
Biomaterials   31(29): 7567-7574 (Journal)
Registered Authors
Wagner, Daniel
Keywords
Theranostics, Cell, Plasmonic nanobubble, Gold nanoparticle, Laser, Cancer
MeSH Terms
  • Animals
  • Cell Line, Tumor
  • Gold/chemistry
  • Humans
  • Male
  • Metal Nanoparticles/chemistry
  • Metal Nanoparticles/therapeutic use
  • Nanostructures/chemistry
  • Nanostructures/therapeutic use*
  • Nanotechnology/methods
  • Prostatic Neoplasms/diagnosis*
  • Prostatic Neoplasms/therapy*
  • Xenograft Model Antitumor Assays
  • Zebrafish
PubMed
20630586 Full text @ Biomaterials
Abstract
Cell theranostics is a new approach that unites diagnosis, therapy and confirmation (guidance) of the results of therapy in one single process at cell level, thus principally improving both the rapidity and precision of treatment. The ideal theranostic agent will support all three of the above functions in vivo with cellular resolution, allowing individual assessment of disease state and the elimination of diseased cells while leaving healthy cells intact. We have developed and evaluated plasmonic nanobubbles (PNBs) as an in vivo tunable theranostic cellular agent in zebrafish hosting prostate cancer xenografts. PNBs were selectively generated around gold nanoparticles in cancer cells in the zebrafish with short single laser pulses. By varying the energy of the laser pulse, we dynamically tuned the PNB size in a theranostic sequence of two PNBs: an initial small PNB detected a cancer cell through optical scattering, followed by a second bigger PNB, which mechanically ablated this cell without damage to surrounding tissue, while its optical scattering confirmed the destruction of the cell. Thus PNBs supported the diagnosis and guided ablation of individual human cancer cells in a living organism without damage to the host.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes