The in vivo performance of plasmonic nanobubbles as cell theranostic agents in zebrafish hosting prostate cancer xenografts
- Wagner, D.S., Delk, N.A., Lukianova-Hleb, E.Y., Hafner, J.H., Farach-Carson, M.C., and Lapotko, D.O.
- Biomaterials 31(29): 7567-7574 (Journal)
- Registered Authors
- Wagner, Daniel
- Theranostics, Cell, Plasmonic nanobubble, Gold nanoparticle, Laser, Cancer
- MeSH Terms
- Cell Line, Tumor
- Metal Nanoparticles/chemistry
- Metal Nanoparticles/therapeutic use
- Nanostructures/therapeutic use*
- Prostatic Neoplasms/diagnosis*
- Prostatic Neoplasms/therapy*
- Xenograft Model Antitumor Assays
- 20630586 Full text @ Biomaterials
Wagner, D.S., Delk, N.A., Lukianova-Hleb, E.Y., Hafner, J.H., Farach-Carson, M.C., and Lapotko, D.O. (2010) The in vivo performance of plasmonic nanobubbles as cell theranostic agents in zebrafish hosting prostate cancer xenografts. Biomaterials. 31(29):7567-7574.
Cell theranostics is a new approach that unites diagnosis, therapy and confirmation (guidance) of the results of therapy in one single process at cell level, thus principally improving both the rapidity and precision of treatment. The ideal theranostic agent will support all three of the above functions in vivo with cellular resolution, allowing individual assessment of disease state and the elimination of diseased cells while leaving healthy cells intact. We have developed and evaluated plasmonic nanobubbles (PNBs) as an in vivo tunable theranostic cellular agent in zebrafish hosting prostate cancer xenografts. PNBs were selectively generated around gold nanoparticles in cancer cells in the zebrafish with short single laser pulses. By varying the energy of the laser pulse, we dynamically tuned the PNB size in a theranostic sequence of two PNBs: an initial small PNB detected a cancer cell through optical scattering, followed by a second bigger PNB, which mechanically ablated this cell without damage to surrounding tissue, while its optical scattering confirmed the destruction of the cell. Thus PNBs supported the diagnosis and guided ablation of individual human cancer cells in a living organism without damage to the host.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes