PUBLICATION
Protein kinase Cgamma is a signaling molecule required for the developmental speeding of alpha-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate receptor kinetics
- Authors
- Patten, S.A., Roy, B., Cunningham, M.E., Stafford, J.L., and Ali, D.W.
- ID
- ZDB-PUB-100614-18
- Date
- 2010
- Source
- The European journal of neuroscience 31(9): 1561-1573 (Journal)
- Registered Authors
- Cunningham, Marcus, Patten, Shumoogum
- Keywords
- glutamate, Mauthner neuron, NMDA, synapse, trafficking, zebrafish
- MeSH Terms
-
- Animals
- Biological Transport, Active/drug effects
- Biological Transport, Active/physiology
- Central Nervous System Agents/pharmacology
- Embryo, Nonmammalian/physiology
- Excitatory Postsynaptic Potentials
- Gene Knockdown Techniques
- Immunohistochemistry
- Kinetics
- N-Methylaspartate/metabolism
- Neurons/drug effects
- Neurons/physiology*
- Patch-Clamp Techniques
- Potassium/metabolism
- Protein Kinase C/genetics
- Protein Kinase C/metabolism*
- Receptors, AMPA/metabolism*
- Rhombencephalon/drug effects
- Rhombencephalon/embryology*
- Rhombencephalon/physiology*
- Signal Transduction
- Synapses/drug effects
- Synapses/physiology*
- Synaptic Transmission/drug effects
- Synaptic Transmission/physiology
- Tetradecanoylphorbol Acetate/analogs & derivatives
- Tetradecanoylphorbol Acetate/pharmacology
- Zebrafish/embryology
- PubMed
- 20525069 Full text @ Eur. J. Neurosci.
Citation
Patten, S.A., Roy, B., Cunningham, M.E., Stafford, J.L., and Ali, D.W. (2010) Protein kinase Cgamma is a signaling molecule required for the developmental speeding of alpha-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate receptor kinetics. The European journal of neuroscience. 31(9):1561-1573.
Abstract
A key step in the maturation of glutamate synapses is the developmental speeding of alpha-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate receptor (AMPA-R) kinetics, which occurs via a switch in receptor subtypes. However, the molecular components required for the switch in receptors are unknown. Here, we used the zebrafish preparation to show that activation of protein kinase C (PKC)gamma is necessary for the developmental speeding of AMPA-R kinetics. Targeted knockdown of PKCgamma with an antisense morpholino oligonucleotide [PKCgamma-morpholino (PKCgamma-MO)], prevents the normal speeding up of AMPA-R kinetics in Mauthner cells. PKCgamma-MO-injected embryos are incapable of trafficking AMPA-Rs following application of phorbol 12-myristate 13-acetate or PKCgamma. PKCgamma-MO-injected embryos do not hatch or exhibit the C-start escape response. Increasing synaptic activity (33 h post-fertilization embryos) by application of an elevated K(+) medium or by application of N-methyl-D-aspartate induces rapid PKCgamma-dependent trafficking of fast AMPA-Rs to synapses. Our findings reveal that PKCgamma is a molecular link underlying the developmental speeding of AMPA-Rs in zebrafish Mauthner cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping