PUBLICATION

Identification and expression analysis of kcnh2 genes in the zebrafish

Authors
Leong, I.U., Skinner, J.R., Shelling, A.N., and Love, D.R.
ID
ZDB-PUB-100511-2
Date
2010
Source
Biochemical and Biophysical Research Communications   396(4): 817-824 (Journal)
Registered Authors
Love, Donald R.
Keywords
Zebrafish, KCNH2, HERG, Zerg, Gene targeting
MeSH Terms
  • Animals
  • Disease Models, Animal
  • Ether-A-Go-Go Potassium Channels/genetics*
  • Gene Expression Regulation
  • Humans
  • Long QT Syndrome/genetics
  • Synteny
  • Transcription, Genetic
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed
20438705 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Long QT syndrome is a disorder that is characterised by a prolonged QT-interval and can lead to fatal cardiac arrhythmias. Many animal models have been created to study congenital long QT syndrome. Of these, zebrafish models have involved targeting two different KCNH2 gene (long QT syndrome 2) orthologues, termed zerg-2 and zerg-3, with differing cardiac phenotypes. In order to clarify this situation, this study uses a bioinformatic approach to search the current zebrafish genome sequence (Zv7 and Zv8 builds) to investigate and locate all likely zebrafish orthologues of the human KCNH2 gene. Quantitative real-time RT-PCR was also used to determine the temporal and spatial gene expression profile of the zebrafish orthologues. The data support the conclusion that zerg-2 and zerg-3 are apparent orthologues of different human genes encoding potassium ion channels, but that their functions have switched compared to the respective human proteins.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping