PUBLICATION
Visualizing Voltage Dynamics in Zebrafish Heart
- Authors
- Tsutsui, H., Higashijima, S.I., Miyawaki, A., and Okamura, Y.
- ID
- ZDB-PUB-100504-2
- Date
- 2010
- Source
- The Journal of physiology 588(Pt 12): 2017-2021 (Journal)
- Registered Authors
- Higashijima, Shin-ichi
- Keywords
- Electrical activity, Fluroscence, Imaging
- MeSH Terms
-
- Action Potentials
- Excitation Contraction Coupling/drug effects
- Heart Rate/drug effects
- Myosin Light Chains/genetics
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/metabolism
- Astemizole/toxicity
- Biosensing Techniques
- Dose-Response Relationship, Drug
- Microscopy, Fluorescence
- Animals, Genetically Modified
- Ether-A-Go-Go Potassium Channels/antagonists & inhibitors
- Ether-A-Go-Go Potassium Channels/metabolism
- Promoter Regions, Genetic
- Kinetics
- Myocytes, Cardiac/drug effects
- Myocytes, Cardiac/metabolism*
- Luminescent Proteins/biosynthesis
- Luminescent Proteins/genetics
- Cardiac Myosins/genetics
- Zebrafish/genetics
- Zebrafish/metabolism*
- Anti-Allergic Agents/toxicity
- Fluorescence Recovery After Photobleaching
- Animals
- PubMed
- 20421282 Full text @ J. Physiol.
Citation
Tsutsui, H., Higashijima, S.I., Miyawaki, A., and Okamura, Y. (2010) Visualizing Voltage Dynamics in Zebrafish Heart. The Journal of physiology. 588(Pt 12):2017-2021.
Abstract
The zebrafish heart provides a useful vertebrate cardiovascular model with outstanding advantages, including genetic manipulatability, optical accessibility and rapid development. In addition, an emerging topic in cardiotoxicity assay and drug discovery is its use in phenotype-based chemical screen. Here, we report a technique that allows non-invasive voltage mapping in beating heart using a genetically encoded probe for transmembrane potential. Application of anti-allergy compound, astemizole, resulted in aberrant propagation of excitation, which accounted for the lack of ventricular contraction. This optical method will provide new opportunities in broad areas of physiological, developmental, and pharmacological cardiovascular research.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping