PUBLICATION
Ethanol exposure affects cell movement during gastrulation and induces split axes in zebrafish embryos
- Authors
- Zhang, Y., Shao, M., Wang, L., Liu, Z., Gao, M., Liu, C., and Zhang, H.
- ID
- ZDB-PUB-100420-37
- Date
- 2010
- Source
- International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 28(4): 283-288 (Journal)
- Registered Authors
- Zhang, Ying
- Keywords
- Ethanol, Split axes, Cell movement, Zebrafish embryo
- MeSH Terms
-
- Animals
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- Body Patterning/drug effects
- Cell Movement/drug effects*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Ethanol/pharmacology*
- Fetal Proteins
- Gastrulation/drug effects*
- Gene Expression Regulation, Developmental/drug effects
- In Situ Hybridization
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Phenotype
- Species Specificity
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 20394815 Full text @ Int. J. Dev. Neurosci.
Citation
Zhang, Y., Shao, M., Wang, L., Liu, Z., Gao, M., Liu, C., and Zhang, H. (2010) Ethanol exposure affects cell movement during gastrulation and induces split axes in zebrafish embryos. International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience. 28(4):283-288.
Abstract
To explore the toxic effects of ethanol on axis formation during embryogenesis, zebrafish embryos at different developmental stages were treated with 3% ethanol for 3hours. The effects of ethanol exposure appeared to be stage-dependent. The dome stage embryo was most sensible to form posterior split axes upon ethanol exposure. Morphological and histological observations and whole-mount in situ hybridization results showed that ethanol exposure at this stage caused a general gastrulation delay, and induced double notochords, double neural tubes and two sets of somites in the posterior trunk. Mechanistically, no ectopic organizer was found by examining the expression patterns of dorsoventral markers including goosecoid, chordin and eve1 at the onset of gastrulation. However, radial intercalation, epiboly and convergence extension were inhibited by ethanol exposure as revealed by cell labeling, phenotypic observation and the expression patterns of axial or paraxial markers. Further investigation showed that the cell aggregation might be affected by ethanol exposure, as indicated by the much more scattered expression pattern of chordin, eve1 and wnt11 at the early gastrula stage, and the discontinuous gsc positive cells during migration. These results imply that ethanol might affect cell movement before and during gastrulation and as a consequence, induces a split axes phenotype.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping