PUBLICATION
Biogenesis of GPI-anchored proteins is essential for surface expression of sodium channels in zebrafish Rohon-Beard neurons to respond to mechanosensory stimulation
- Authors
- Nakano, Y., Fujita, M., Ogino, K., Saint-Amant, L., Kinoshita, T., Oda, Y., and Hirata, H.
- ID
- ZDB-PUB-100420-35
- Date
- 2010
- Source
- Development (Cambridge, England) 137(10): 1689-1698 (Journal)
- Registered Authors
- Hirata, Hiromi, Oda, Yoichi, Saint-Amant, Louis
- Keywords
- Zebrafish, Sodium channel, Behavior, Rohon-Beard neuron, GPI-anchored protein, GPI transamidase, Touch response
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Antigens, Surface/metabolism
- CHO Cells
- Cell Death/drug effects
- Cell Death/genetics
- Cell Death/physiology
- Cricetinae
- Cricetulus
- Embryo, Nonmammalian
- Gene Knockdown Techniques
- Glycosylphosphatidylinositols/biosynthesis
- Glycosylphosphatidylinositols/metabolism*
- Mechanotransduction, Cellular/genetics*
- Membrane Proteins/biosynthesis*
- Membrane Proteins/metabolism
- Membrane Proteins/physiology
- Physical Stimulation
- RNA, Small Interfering/pharmacology
- Sensory Receptor Cells/drug effects
- Sensory Receptor Cells/metabolism*
- Sensory Receptor Cells/physiology
- Sodium Channels/metabolism*
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 20392743 Full text @ Development
Citation
Nakano, Y., Fujita, M., Ogino, K., Saint-Amant, L., Kinoshita, T., Oda, Y., and Hirata, H. (2010) Biogenesis of GPI-anchored proteins is essential for surface expression of sodium channels in zebrafish Rohon-Beard neurons to respond to mechanosensory stimulation. Development (Cambridge, England). 137(10):1689-1698.
Abstract
In zebrafish, Rohon-Beard (RB) neurons are primary sensory neurons present during the embryonic and early larval stages. At 2 days post-fertilization (dpf), wild-type zebrafish embryos respond to mechanosensory stimulation and swim away from the stimuli, whereas mi310 mutants are insensitive to touch. During approximately 2-4 dpf, wild-type RB neurons undergo programmed cell death, which is caused by sodium current-mediated electrical activity, whereas mutant RB cells survive past 4 dpf, suggesting a defect of sodium currents in the mutants. Indeed, electrophysiological recordings demonstrated the generation of action potentials in wild-type RB neurons, whereas mutant RB cells failed to fire owing to the reduction of voltage-gated sodium currents. Labeling of dissociated RB neurons with an antibody against voltage-gated sodium channels revealed that sodium channels are expressed at the cell surface in wild-type, but not mutant, RB neurons. Finally, in mi310 mutants, we identified a mis-sense mutation in pigu, a subunit of GPI (glycosylphosphatidylinositol) transamidase, which is essential for membrane anchoring of GPI-anchored proteins. Taken together, biogenesis of GPI-anchored proteins is necessary for cell surface expression of sodium channels and thus for firings of RB neurons, which enable zebrafish embryos to respond to mechanosensory stimulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping