PUBLICATION
Oligonucleotide-Based Tools for Studying Zebrafish Development
- Authors
- Shestopalov, I.A., and Chen, J.K.
- ID
- ZDB-PUB-100420-32
- Date
- 2010
- Source
- Zebrafish 7(1): 31-40 (Review)
- Registered Authors
- Chen, James K., Shestopalov, Ilya
- Keywords
- none
- MeSH Terms
-
- Animals
- Gene Expression Regulation, Developmental
- Genetic Techniques*
- Oligonucleotides/genetics*
- RNA/genetics
- Zebrafish/genetics*
- Zebrafish/growth & development*
- PubMed
- 20392138 Full text @ Zebrafish
Citation
Shestopalov, I.A., and Chen, J.K. (2010) Oligonucleotide-Based Tools for Studying Zebrafish Development. Zebrafish. 7(1):31-40.
Abstract
Synthetic and nonnatural oligonucleotides have been used extensively to interrogate gene function in zebrafish. In this review, we survey the capabilities and limitations of various oligonucleotide-based technologies for perturbing RNA function and tracking RNA expression. We also examine recent strategies for achieving spatiotemporal control of oligonucleotide function, particularly light-gated technologies that exploit the optical transparency of zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping