PUBLICATION

Oligonucleotide-Based Tools for Studying Zebrafish Development

Authors
Shestopalov, I.A., and Chen, J.K.
ID
ZDB-PUB-100420-32
Date
2010
Source
Zebrafish   7(1): 31-40 (Review)
Registered Authors
Chen, James K., Shestopalov, Ilya
Keywords
none
MeSH Terms
  • Animals
  • Gene Expression Regulation, Developmental
  • Genetic Techniques*
  • Oligonucleotides/genetics*
  • RNA/genetics
  • Zebrafish/genetics*
  • Zebrafish/growth & development*
PubMed
20392138 Full text @ Zebrafish
Abstract
Synthetic and nonnatural oligonucleotides have been used extensively to interrogate gene function in zebrafish. In this review, we survey the capabilities and limitations of various oligonucleotide-based technologies for perturbing RNA function and tracking RNA expression. We also examine recent strategies for achieving spatiotemporal control of oligonucleotide function, particularly light-gated technologies that exploit the optical transparency of zebrafish embryos.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping