Oligonucleotide-Based Tools for Studying Zebrafish Development
- Shestopalov, I.A., and Chen, J.K.
- Zebrafish 7(1): 31-40 (Review)
- Registered Authors
- Chen, James K., Shestopalov, Ilya
- MeSH Terms
- Gene Expression Regulation, Developmental
- Genetic Techniques*
- Zebrafish/growth & development*
- 20392138 Full text @ Zebrafish
Shestopalov, I.A., and Chen, J.K. (2010) Oligonucleotide-Based Tools for Studying Zebrafish Development. Zebrafish. 7(1):31-40.
Synthetic and nonnatural oligonucleotides have been used extensively to interrogate gene function in zebrafish. In this review, we survey the capabilities and limitations of various oligonucleotide-based technologies for perturbing RNA function and tracking RNA expression. We also examine recent strategies for achieving spatiotemporal control of oligonucleotide function, particularly light-gated technologies that exploit the optical transparency of zebrafish embryos.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes