PUBLICATION

Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb

Authors
Mao, J., McGlinn, E., Huang, P., Tabin, C.J., and McMahon, A.P.
ID
ZDB-PUB-100406-1
Date
2009
Source
Developmental Cell   16(4): 600-606 (Journal)
Registered Authors
Huang, Peng, McMahon, Andrew
Keywords
DEVBIO
MeSH Terms
  • Animals
  • Body Patterning*
  • Cell Polarity
  • Cells, Cultured
  • Chick Embryo
  • DNA-Binding Proteins/metabolism*
  • Ectoderm/cytology
  • Ectoderm/embryology
  • Ectoderm/metabolism
  • Extremities/embryology*
  • Fibroblast Growth Factor 8/metabolism*
  • Hedgehog Proteins/metabolism*
  • Integrases/metabolism
  • Mesoderm/cytology
  • Mesoderm/embryology
  • Mesoderm/metabolism
  • Mice
  • Paired Box Transcription Factors/metabolism
  • Polydactyly/pathology
  • Proto-Oncogene Proteins c-ets/metabolism*
  • Repressor Proteins
  • Transcription Factors/metabolism*
  • Vertebrates/embryology*
PubMed
19386268 Full text @ Dev. Cell
Abstract
Crosstalk between the fibroblast growth factor (FGF) and Sonic Hedgehog (Shh) pathways is critical for proper patterning and growth of the developing limb bud. Here, we show that FGF-dependent activation of the ETS transcription factors Etv4 and Etv5 contributes to proximal-distal limb outgrowth. Surprisingly, blockage of Etv activity in early distal mesenchyme also resulted in ectopic, anterior expansion of Shh, leading to a polydactylous phenotype. These data indicate an unexpected function for an FGF/Etv pathway in anterior-posterior patterning. FGF activity in the limb is not only responsible for maintaining posterior-specific Shh expression, but it also acts via Etvs to prevent inappropriate anterior expansion of Shh. This study identifies another level of genetic interaction between the orthogonal axes during limb development.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes