PUBLICATION
Rapid Degeneration and Regeneration of the Zebrafish Olfactory Epithelium after Triton X-100 Application
- Authors
- Iqbal, T., and Byrd-Jacobs, C.
- ID
- ZDB-PUB-100317-32
- Date
- 2010
- Source
- Chemical senses 35(5): 351-361 (Journal)
- Registered Authors
- Keywords
- chemical lesion, immunocytochemistry, neuronal turnover, olfactory sensory neurons, teleost
- MeSH Terms
-
- Animals
- Apoptosis/drug effects
- Cells, Cultured
- Detergents/pharmacology*
- Neurons/drug effects*
- Neurons/pathology
- Octoxynol/pharmacology*
- Olfactory Bulb/cytology*
- Olfactory Bulb/drug effects
- Olfactory Mucosa/drug effects*
- Olfactory Mucosa/pathology
- Regeneration
- Zebrafish
- PubMed
- 20228140 Full text @ Chem. Senses
Citation
Iqbal, T., and Byrd-Jacobs, C. (2010) Rapid Degeneration and Regeneration of the Zebrafish Olfactory Epithelium after Triton X-100 Application. Chemical senses. 35(5):351-361.
Abstract
The effects of Triton X-100 on the olfactory epithelium (OE) of adult zebrafish were examined to study neuronal turnover in this model system. Fish were killed at various time points after detergent application and stained with hematoxylin and eosin to examine olfactory structures, immunocytochemistry to examine cell types, or DiI to examine connections to the olfactory bulb. A significant decrease in epithelial thickness of treated sides was observed 1-day posttreatment. Epithelium thickness recovered by 5 days. The most significant reduction in the OE following Triton X-100 treatment corresponded to the region of supporting cells and mature olfactory sensory neurons. Labeling for all neurons with anti-Hu and for the 3 sensory neuron subtypes of the zebrafish OE (ciliated, microvillous, and crypt neurons) diminished 1 day after lesion and returned by 5 days posttreatment. Retrograde labeling from the olfactory bulb showed that the majority of mature olfactory sensory neurons disappeared in 1 day and reappeared by 5 days after treatment. Anti-proliferating cell nuclear antigen was used to show mitotic activity, and after chemical lesion, there was an increase in proliferation in specific regions of the OE. Thus, chemical ablation causes temporary reduction with swift regeneration of the OE occurring within a week.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping