PUBLICATION
Haemostasis in Danio rerio: Is the zebrafish a useful model for platelet research?
- Authors
- Lang, M.R., Gihr, G., Gawaz, M.P., and Müller, I.I.
- ID
- ZDB-PUB-100302-7
- Date
- 2010
- Source
- Journal of thrombosis and haemostasis : JTH 8(6): 1159-1169 (Review)
- Registered Authors
- Lang, Michael
- Keywords
- haemostasis, mutagenesis, platelet, thrombocyte, thrombosis, zebrafish
- MeSH Terms
-
- Animals
- Disease Models, Animal*
- Hemostasis*
- Zebrafish/metabolism*
- PubMed
- 20180901 Full text @ J. Thromb. Haemost.
Citation
Lang, M.R., Gihr, G., Gawaz, M.P., and Müller, I.I. (2010) Haemostasis in Danio rerio: Is the zebrafish a useful model for platelet research?. Journal of thrombosis and haemostasis : JTH. 8(6):1159-1169.
Abstract
To better understand platelet function and to develop new preventive and therapeutic options, new scientific models have been established in the last years to identify new factors of haemostasis and thrombosis and to analyse their function in greater detail. One fairly new animal model is the zebrafish, Danio rerio, which shares most of the central factors of platelet adhesion, activation, aggregation and release reaction with humans. Examples include GPIIbIIIa, many other integrins, coagulation factors, inflammatory and cytokine-like proteins as well as arachidonic acid metabolism enzymes. Yet the zebrafish genome has undergone a teleost-specific genome duplication, causing the existence of duplicated paralogues in some instances, and a few genes have not been identified in the zebrafish genome. Taken together the high fecundity of the zebrafish, the possibility to observe transparent developing embryos in real time, the availability of a large number of mutants and transgenics as well as the possibility to knock down gene function by microinjection of morpholino antisense oligonucleotides and the similarity of the hemostatic system are important assets of the zebrafish, promising that it will be an attractive model to study thrombocyte function, thrombosis and haemostasis. This review provides an overview of the central factors of thrombocyte function identified so far in the zebrafish genome and a compilation of methods and tools available for the study of thrombocyte development and function in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping