ZFIN ID: ZDB-PUB-100223-4
Culturing and Transfecting Zebrafish PAC2 Fibroblast Cells
Senghaas, N., and Köster, R.W.
Date: 2009
Source: CSH protocols   2009(6): pdb.prot5235 (Journal)
Registered Authors: Köster, Reinhard W., Senghaas, Niklas
Keywords: none
MeSH Terms:
  • Animals
  • Cell Culture Techniques/methods*
  • Cell Line
  • Cell Survival
  • Cryopreservation
  • Fibroblasts/cytology*
  • Immunohistochemistry
  • Tissue Fixation
  • Transfection/methods*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/isolation & purification
PubMed: 20147193 Full text @ CSH Protoc.
INTRODUCTION The use of zebrafish (Danio rerio) as a model organism has seen rapid growth in recent years. Numerous new techniques have emerged for zebrafish, and many protocols used in other model organisms have been adapted to fit the special needs of this species. Zebrafish cell lines, however, have not been studied to a great extent, and their applications remain limited. The PAC2 fibroblast line, isolated from 24-h post-fertilization zebrafish embryos, is one of the few available lines. Here, we provide a basic set of methods for maintenance and handling of PAC2 cells, as well as general procedures for transfection, immunocytochemistry, and protein extraction.