ZFIN ID: ZDB-PUB-100211-23
MAZe: a tool for mosaic analysis of gene function in zebrafish
Collins, R.T., Linker, C., and Lewis, J.
Date: 2010
Source: Nature Methods   7(3): 219-223 (Journal)
Registered Authors: Lewis, Julian
Keywords: none
MeSH Terms:
  • Animals
  • Base Sequence
  • Cell Fusion
  • HSP70 Heat-Shock Proteins/genetics
  • Integrases/physiology
  • Molecular Sequence Data
  • Mosaicism*
  • Myoblasts/metabolism
  • Organ Specificity
  • Promoter Regions, Genetic
  • Recombination, Genetic
  • Transgenes*
  • Zebrafish/genetics*
PubMed: 20139970 Full text @ Nat. Methods
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ABSTRACT
To trace cell lineages in a developing vertebrate and to observe, in vivo, how behaviors of individual cells are affected by the genes they express, we created a zebrafish line containing a transgene called mosaic analysis in zebrafish (MAZe), built around a self-excising hsp70:Cre cassette. Heat shock triggers Cre recombinase-mediated recombination in a random subset of cells, bringing the transcriptional activator Gal4:VP16 under control of the EF1alpha promoter. Gal4-VP16 then activates expression of a fluorescent protein from an upstream activating sequence (UAS) promoter. Marked clones of cells expressing any desired gene product can be generated by crossing MAZe fish with other lines containing UAS-driven transgenes. The number of clones induced, and their time of origin, could be varied by adjusting heat-shock timing and duration. As an alternative to heat shock, we introduced Cre under a tissue-specific promoter in MAZe fish to generate clones in a designated tissue.
ADDITIONAL INFORMATION