PUBLICATION
Loss of GATA1 and gain of FLI1 expression during thrombocyte maturation
- Authors
- Jagadeeswaran, P., Lin, S., Weinstein, B., Hutson, A., and Kim, S.
- ID
- ZDB-PUB-100202-13
- Date
- 2010
- Source
- Blood cells, molecules & diseases 44(3): 175-180 (Journal)
- Registered Authors
- Hutson, Angela, Jagadeeswaran, Pudur, Kim, Seongcheol, Lin, Shuo, Weinstein, Brant M.
- Keywords
- Zebrafish, Thrombocyte, Transcription, Maturation, GFP, FLI1, GATA1
- MeSH Terms
-
- Thrombosis
- Platelet Aggregation
- Animals
- Zebrafish Proteins/genetics*
- Animals, Genetically Modified
- Proto-Oncogene Protein c-fli-1/genetics*
- Blood Platelets/cytology*
- Blood Platelets/metabolism
- Gene Expression Regulation*
- Zebrafish/genetics*
- Zebrafish/metabolism
- Thrombopoiesis
- GATA1 Transcription Factor/genetics*
- PubMed
- 20110178 Full text @ Blood Cells Mol. Dis.
Citation
Jagadeeswaran, P., Lin, S., Weinstein, B., Hutson, A., and Kim, S. (2010) Loss of GATA1 and gain of FLI1 expression during thrombocyte maturation. Blood cells, molecules & diseases. 44(3):175-180.
Abstract
In this paper, we characterized expression of GATA1 and FLI1 gene promoters in thrombocytes of zebrafish transgenic lines, G1-GM2 and TG(fli1:EGFP)y1 that carry transgenes of GATA1 and FLI1 gene promoters driving GFP. We found two discrete populations of thrombocytes verified by morphology, labeled with GFP in both G1-GM2 and TG(fli1:EGFP)y1 lines: (1) the more intensely labeled GFP+ thrombocyte, and (2) the less intensely labeled GFP+ thrombocytes. The more intensely labeled GFP+ thrombocyte in G1-GM2 line and the less intensely labeled GFP+ thrombocytes in the TG(fli1:EGFP)y1 line corresponded to young thrombocytes. These results showed that young thrombocytes have higher GATA1 promoter activity, while mature thrombocytes have more FLI1 gene promoter transcription. This finding suggests that there is a gradual loss of GATA1 and gain of FLI1 expression as the thrombocytes mature, and this overexpression of FLI1 may help maintain the thrombocyte lineage. Furthermore, the presence of transcriptional factors similar to those found in megakaryocytes raises the possibility that vertebrate thrombocytes may be the forerunners of mammalian megakaryocytes and, therefore, could serve as a model to study megakaryocyte maturation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping