PUBLICATION
Leptin: Clue to poor appetite in oxygen-starved fish
- Authors
- Chu, D.L., Li, V.W., and Yu, R.M.
- ID
- ZDB-PUB-100126-2
- Date
- 2010
- Source
- Molecular and Cellular Endocrinology 319(1-2): 143-146 (Journal)
- Registered Authors
- Keywords
- Feeding, Hypoxia, Hypoxia-inducible factor, Leptin, Zebrafish
- MeSH Terms
-
- Animals
- Gene Expression Regulation/genetics
- Hypoxia/genetics
- Hypoxia/metabolism*
- Hypoxia-Inducible Factor 1, alpha Subunit/genetics
- Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
- Leptin/genetics
- Leptin/metabolism*
- Liver/metabolism*
- Promoter Regions, Genetic
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 20085798 Full text @ Mol. Cell. Endocrinol.
Citation
Chu, D.L., Li, V.W., and Yu, R.M. (2010) Leptin: Clue to poor appetite in oxygen-starved fish. Molecular and Cellular Endocrinology. 319(1-2):143-146.
Abstract
Hypoxia is the most widespread deleterious consequence of eutrophication and has become a major cause of fishery decline. One feature of chronic exposure to hypoxia in fish is inhibition of feeding. In this study, we investigated if the gene that encodes the appetite-suppressing hormone leptin is regulated by hypoxia in zebrafish (Danio rerio). Exposure of adult zebrafish to hypoxic conditions (1+/-0.2mg O(2) L(-1)) for 4 and 10 days significantly increased leptin-a (zlep-a) mRNA levels in the liver. To evaluate the role of hypoxia-inducible factor 1 (HIF-1) in regulating zlep-a expression, zebrafish embryos were exposed to cobalt chloride (CoCl(2), a HIF-1 inducer) and overexpressed with HIF-1alpha mRNA. Both CoCl(2) treatment and HIF-1alpha overexpression markedly increased zlep-a expression in developing embryos, indicating the possible involvement of HIF-1 in zlep-a regulation. In vivo promoter analysis indicated that zlep-a promoter activity is found in the muscle fibers of zebrafish embryos and enhanced by CoCl(2). This is the first report to show that leptin gene expression in fish is regulated by hypoxia possibly via the involvement of HIF-1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping