PUBLICATION
Growth and transcriptional effect of dietary 2,2',4,4'-tetrabromodiphenyl ether (PBDE-47) exposure in developing zebrafish (Danio rerio)
- Authors
- Chen, T.H., Cheng, Y.M., Cheng, J.O., Chou, C.T., Hsiao, Y.C., and Ko, F.C.
- ID
- ZDB-PUB-100119-10
- Date
- 2010
- Source
- Ecotoxicology and environmental safety 73(3): 377-383 (Journal)
- Registered Authors
- Keywords
- Polybrominated diphenyl ethers, Brominated flame retardant, Zebrafish, Gene expression
- MeSH Terms
-
- Animal Feed
- Animals
- Body Size/drug effects
- Cytochrome P-450 CYP1A1/genetics
- Cytochrome P-450 CYP1A1/metabolism
- Female
- Gene Expression Profiling
- Gene Expression Regulation, Enzymologic/drug effects
- Halogenated Diphenyl Ethers/metabolism
- Halogenated Diphenyl Ethers/toxicity*
- Life Cycle Stages/drug effects*
- Life Cycle Stages/physiology
- Longevity/drug effects
- Male
- RNA, Messenger/metabolism
- Toxicity Tests
- Transcription, Genetic/drug effects*
- Water Pollutants, Chemical/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/physiology*
- PubMed
- 20074802 Full text @ Ecotoxicol. Environ. Saf.
Citation
Chen, T.H., Cheng, Y.M., Cheng, J.O., Chou, C.T., Hsiao, Y.C., and Ko, F.C. (2010) Growth and transcriptional effect of dietary 2,2',4,4'-tetrabromodiphenyl ether (PBDE-47) exposure in developing zebrafish (Danio rerio). Ecotoxicology and environmental safety. 73(3):377-383.
Abstract
In this study, zebrafish (Danio rerio) were fed food dosed with pure PBDE-47 (2,2',4,4'-tetrabromodiphenyl ether) congener or a blank from 20 to 60 day post-hatch (dph). At 38dph, half of the fish were sampled for body size measurements and gene expression analyzes (CYP1A1, VTG, TTR, D1, and TSH-beta). At 60dph, body size was measured again for all fish remaining. Whole-fish histology was performed and the PBDE levels in fish were determined. PBDE treated fish was significantly smaller at 38dph but not at 60dph. No apparent histopathological effect was observed. In the PBDE treated fish, there was a weak induction of CYP1A1 mRNA transcription, but not of the other genes. The tissue levels of PBDE-47 were comparable to that found in other wild fish reported in the literature, indicating that our exposure level was ecologically relevant.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping