ZFIN ID: ZDB-PUB-091215-17
Exposure to DE-71 alters thyroid hormone levels and gene transcription in the hypothalamic-pituitary-thyroid axis of zebrafish larvae
Yu, L., Deng, J., Shi, X., Liu, C., Yu, K., and Zhou, B.
Date: 2010
Source: Aquatic toxicology (Amsterdam, Netherlands)   97(3): 226-233 (Journal)
Registered Authors:
Keywords: PBDEs, Hypothalamic–pituitary–thyroid axis, Gene expression, Thyroid hormone, Zebrafish
MeSH Terms:
  • Animals
  • Dose-Response Relationship, Drug
  • Flame Retardants/toxicity*
  • Halogenated Diphenyl Ethers/toxicity*
  • Hypothalamo-Hypophyseal System/drug effects
  • Hypothalamo-Hypophyseal System/metabolism
  • Larva
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Thyroid Gland/drug effects
  • Thyroid Gland/metabolism
  • Thyroid Hormones/metabolism*
  • Transcription, Genetic/drug effects*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 19945756 Full text @ Aquat. Toxicol.
Polybrominated diphenyl ethers (PBDEs) have the potential to disrupt thyroid hormone homeostasis, but the molecular mechanisms underlying this process have not yet been clarified. In the present study, zebrafish (Danio rerio) embryos were exposed to a low concentration (0, 1, 3, and 10mug/L) of DE-71 from fertilization to 14 days thereafter. The whole-body content of thyroid hormone and transcription of genes in the hypothalamic-pituitary-thyroid (HPT) axis were analyzed. Exposure to up to 10mug/L of DE-71 significantly reduced thyroxine (T4) levels and significantly upregulated the transcription of corticotrophin-releasing hormone (CRH) and thyroid-stimulating hormone (TSHbeta) genes in a concentration-dependent manner. The transcription of genes involved in the synthesis of TH proteins, sodium/iodide symporter (Slc5a5), and thyroglobulin (TG) and the transcription of marker genes associated with early thyroid development (Pax8 and Nkx2.1) were significantly upregulated upon DE-71 exposure. The expression of thyronine deiodinase (Deio1 and Deio2) mRNAs was also significantly upregulated, possibly as a compensatory response to the decreased T4 levels. However, DE-71 exposure resulted in the downregulation of transthyretin (TTR) gene transcription and did not affect the transcription of thyroid hormone receptors (TRs). Exposure to DE-71 significantly induced the transcription of the uridinediphosphate-glucuronosyltransferase (UGT1ab) gene. The results of our study confirmed the reliability of the zebrafish larvae as models for assessment of the developmental toxicity of PBDEs and transcription of genes of the HPT axis can evaluate the potential mechanisms of thyroid disruption.
Erratum in: Aquat. Toxicol. 2010 November: 100(4):376
The corresponding author apologized for using a wrong primer for thyroid peroxidase (TPO) in the above article. Please find that this primer should be for thrombopoeitin.