ZFIN ID: ZDB-PUB-091204-9
Tsukushi functions as an organizer inducer by inhibition of BMP activity in cooperation with chordin
Ohta, K., Lupo, G., Kuriyama, S., Keynes, R., Holt, C.E., Harris, W.A., Tanaka, H., and Ohnuma, S.
Date: 2004
Source: Developmental Cell   7(3): 347-358 (Journal)
Registered Authors: Harris, William A., Tanaka, Hideaki
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Blotting, Western
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins/metabolism*
  • Chick Embryo
  • Cloning, Molecular
  • Gastrula/cytology
  • Gene Expression Regulation, Developmental*
  • Gene Library
  • Glycoproteins/metabolism*
  • In Situ Hybridization
  • Intercellular Signaling Peptides and Proteins/metabolism*
  • Lens, Crystalline/embryology
  • Molecular Sequence Data
  • Neurons/metabolism
  • Protein Binding
  • Proteoglycans/physiology*
  • RNA, Messenger/metabolism
  • RNA, Small Interfering/metabolism
  • Sequence Homology, Amino Acid
  • Xenopus
  • Xenopus Proteins
PubMed: 15363410 Full text @ Dev. Cell
During chick gastrulation, inhibition of BMP signaling is required for primitive streak formation and induction of Hensen's node. We have identified a unique secreted protein, Tsukushi (TSK), which belongs to the Small Leucine-Rich Proteoglycan (SLRP) family and is expressed in the primitive streak and Hensen's node. Grafts of cells expressing TSK in combination with the middle primitive streak induce an ectopic Hensen's node, while electroporation of TSK siRNA inhibits induction of the node. In Xenopus embryos, TSK can block BMP function and induce a secondary dorsal axis, while it can dorsalize ventral mesoderm and induce neural tissue in embryonic explants. Biochemical analysis shows that TSK binds directly to both BMP and chordin and forms a ternary complex with them. These observations indicate that TSK is an essential dorsalizing factor involved in the induction of Hensen's node.