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ZFIN ID: ZDB-PUB-091023-30
Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation
Herbert, S.P., Huisken, J., Kim, T.N., Feldman, M.E., Houseman, B.T., Wang, R.A., Shokat, K.M., and Stainier, D.Y.
Date: 2009
Source: Science (New York, N.Y.)   326(5950): 294-298 (Journal)
Registered Authors: Stainier, Didier
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Aorta/cytology
  • Aorta/embryology
  • Arteries/cytology
  • Arteries/embryology*
  • Cell Movement
  • Endothelial Cells/cytology
  • Endothelial Cells/physiology*
  • Ephrin-B2/metabolism*
  • Morphogenesis*
  • Phosphatidylinositol 3-Kinases/metabolism
  • Receptor, EphB4/metabolism*
  • Receptors, Notch/metabolism
  • Signal Transduction
  • Stem Cells/cytology
  • Stem Cells/physiology*
  • Vascular Endothelial Growth Factor A/metabolism
  • Vascular Endothelial Growth Factor Receptor-2/metabolism
  • Vascular Endothelial Growth Factor Receptor-3/metabolism
  • Veins/cytology
  • Veins/embryology*
  • Zebrafish
  • Zebrafish Proteins/metabolism
PubMed: 19815777 Full text @ Science
Blood vessels form de novo (vasculogenesis) or upon sprouting of capillaries from preexisting vessels (angiogenesis). With high-resolution imaging of zebrafish vascular development, we uncovered a third mode of blood vessel formation whereby the first embryonic artery and vein, two unconnected blood vessels, arise from a common precursor vessel. The first embryonic vein formed by selective sprouting of progenitor cells from the precursor vessel, followed by vessel segregation. These processes were regulated by the ligand EphrinB2 and its receptor EphB4, which are expressed in arterial-fated and venous-fated progenitors, respectively, and interact to orient the direction of progenitor migration. Thus, directional control of progenitor migration drives arterial-venous segregation and generation of separate parallel vessels from a single precursor vessel, a process essential for vascular development.