ZFIN ID: ZDB-PUB-091023-14
Zebrafish CiA interneurons are late-born primary neurons
Yeo, S.Y.
Date: 2009
Source: Neuroscience letters   466(3): 131-134 (Journal)
Registered Authors: Yeo, Sang-Yeob
Keywords: Notch, Zebrafish, Pax2, CiA, Primary neurons, Late-born neurons, Mind bomb, CoPA
MeSH Terms:
  • Animals
  • Embryo, Nonmammalian
  • Gene Knockdown Techniques
  • Immunohistochemistry
  • Interneurons/cytology*
  • Interneurons/metabolism
  • Mutation
  • Neurons/cytology*
  • Neurons/metabolism
  • PAX2 Transcription Factor/biosynthesis
  • RNA, Messenger/genetics
  • Receptors, Notch/genetics
  • Spinal Cord/cytology
  • Spinal Cord/embryology
  • Spinal Cord/metabolism
  • Ubiquitin-Protein Ligases/genetics
  • Xenopus
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics
PubMed: 19800937 Full text @ Neurosci. Lett.
Pax2 is a neural-related transcription factor downstream of Notch signaling and is expressed in the developing spinal cord of zebrafish, including in CiA interneurons. However, the characteristics of pax2-positive neurons are largely unknown. The goal of this study was to characterize Pax2-positive neurons by examining their expression in embryos in which Notch function had been knocked down by mutation or injection of a morpholino or mRNA. I found that Pax2-positive CiA interneurons were late-differentiating primary neurons. pax2.1 was expressed in CoPA commissural neurons and CiA interneurons at 26 hpf. The number of pax2.1-positive cells increased in mind bomb mutant embryos or embryos injected with Su(H)1-MO, but not in cells injected with Xenopus Delta or Delta(stu) mRNA. These observations imply that Notch signaling plays a role in regulating the number of CiA neurons by preventing uncommitted precursors from acquiring a neuronal fate during vertebrate development.