|ZFIN ID: ZDB-PUB-091023-14|
Zebrafish CiA interneurons are late-born primary neurons
|Source:||Neuroscience letters 466(3): 131-134 (Journal)|
|Registered Authors:||Yeo, Sang-Yeob|
|Keywords:||Notch, Zebrafish, Pax2, CiA, Primary neurons, Late-born neurons, Mind bomb, CoPA|
|PubMed:||19800937 Full text @ Neurosci. Lett.|
Yeo, S.Y. (2009) Zebrafish CiA interneurons are late-born primary neurons. Neuroscience letters. 466(3):131-134.
ABSTRACTPax2 is a neural-related transcription factor downstream of Notch signaling and is expressed in the developing spinal cord of zebrafish, including in CiA interneurons. However, the characteristics of pax2-positive neurons are largely unknown. The goal of this study was to characterize Pax2-positive neurons by examining their expression in embryos in which Notch function had been knocked down by mutation or injection of a morpholino or mRNA. I found that Pax2-positive CiA interneurons were late-differentiating primary neurons. pax2.1 was expressed in CoPA commissural neurons and CiA interneurons at 26 hpf. The number of pax2.1-positive cells increased in mind bomb mutant embryos or embryos injected with Su(H)1-MO, but not in cells injected with Xenopus Delta or Delta(stu) mRNA. These observations imply that Notch signaling plays a role in regulating the number of CiA neurons by preventing uncommitted precursors from acquiring a neuronal fate during vertebrate development.