PUBLICATION
Introduction of a Foreign Gene into Zebrafish and Medaka Cells Using Adenoviral Vectors
- Authors
- Kawasaki, T., Saito, K., Mitsui, K., Ikawa, M., Yamashita, M., Taniguchi, Y., Takeda, S., Mitani, K., and Sakai, N.
- ID
- ZDB-PUB-090914-9
- Date
- 2009
- Source
- Zebrafish 6(3): 253-258 (Journal)
- Registered Authors
- Sakai, Noriyoshi
- Keywords
- none
- MeSH Terms
-
- Adenoviridae/genetics*
- Animals
- Cells, Cultured
- Genes, Reporter
- Genetic Vectors*
- Lentivirus/genetics
- Male
- Oryzias/genetics*
- Oryzias/metabolism
- Testis/metabolism
- Testis/virology
- Transfection/methods*
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 19715489 Full text @ Zebrafish
Citation
Kawasaki, T., Saito, K., Mitsui, K., Ikawa, M., Yamashita, M., Taniguchi, Y., Takeda, S., Mitani, K., and Sakai, N. (2009) Introduction of a Foreign Gene into Zebrafish and Medaka Cells Using Adenoviral Vectors. Zebrafish. 6(3):253-258.
Abstract
Viral vectors represent a tractable system that can efficiently introduce an exogenous gene into different target cells and are thus a potentially powerful genetic manipulation tool. In our current study, we investigated the infection efficiency of mammalian virus vectors, adenoviruses (Ads), adeno-associated viruses, and lentiviruses to the Sertoli cell line and the newly established cell line from a single embryo in zebrafish. Among the viral vectors tested, Ads showed the highest infection efficiency of 10(7)-10(8) green fluorescent protein-transducing units (gtu)/mL in zebrafish cells. In addition, the adenoviral vector was also infected at 10(5) gtu/mL in the medaka testicular somatic cell line that was established from the testes of p53-deficient mutant. Further, we found that Ads could successfully infect cultured male zebrafish germ cells. Our results thus indicate that the adenoviral vector could be used as a chromosomally nonintegrating vector system in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping