PUBLICATION
TC1(C8orf4) is a novel endothelial inflammatory regulator enhancing NF-kappaB activity
- Authors
- Kim, J., Kim, Y., Kim, H.T., Kim, D.W., Ha, Y., Kim, J., Kim, C.H., Lee, I., and Song, K.
- ID
- ZDB-PUB-090914-4
- Date
- 2009
- Source
- Journal of immunology (Baltimore, Md. : 1950) 183(6): 3996-4002 (Journal)
- Registered Authors
- Kim, Cheol-Hee, Kim, Hyun-Taek
- Keywords
- none
- MeSH Terms
-
- Active Transport, Cell Nucleus
- Animals
- Cells, Cultured
- Embryo, Nonmammalian
- Endothelium, Vascular/pathology*
- Humans
- Inflammation/etiology*
- Mice
- NF-kappa B/metabolism*
- Neoplasm Proteins/physiology*
- Transcription Factor RelA/metabolism
- Up-Regulation
- Zebrafish
- PubMed
- 19684084 Full text @ J. Immunol.
Citation
Kim, J., Kim, Y., Kim, H.T., Kim, D.W., Ha, Y., Kim, J., Kim, C.H., Lee, I., and Song, K. (2009) TC1(C8orf4) is a novel endothelial inflammatory regulator enhancing NF-kappaB activity. Journal of immunology (Baltimore, Md. : 1950). 183(6):3996-4002.
Abstract
Endothelial inflammation is regulated by a complex molecular mechanism. TC1(C8orf4) is a novel regulator implicated in cancer and inflammation. It is a small protein conserved well among vertebrates. In zebrafish embryos, it is mostly expressed in angio-hematopoietic system and the overexpression induces edema. In human aortic endothelial cells and umbilical vein endothelial cells, TC1 transfection up-regulates key inflammatory cytokines, enzymes, and adhesion proteins including IL-6, IL-1alpha, COX-2, CXCL1, CCL5, CCL2, IL-8, ICAM1, VCAM1, and E-selectin, while TC1 knockdown down-regulates them. TC1 also enhances inflammatory parameters such as monocyte-endothelial adhesion and endothelial monolayer permeability. TC1 is up-regulated by IL-1beta, TNF-alpha, LPS, and phorbol ester, and the up-regulation is inhibited by I-kappaB-kinase inhibitors. TC1, in turn, enhances the nuclear translocation of RelA and the DNA binding activity, suggesting a biological role of amplifying NF-kappaB signaling via a positive feedback. Our findings suggest that TC1 is a novel endothelial inflammatory regulator that might be implicated in inflammatory vascular diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping