PUBLICATION
Distinct populations of quiescent and proliferative pancreatic β-cells identified by HOTcre mediated labeling
- Authors
- Hesselson, D., Anderson, R.M., Beinat, M., and Stainier, D.Y.
- ID
- ZDB-PUB-090828-26
- Date
- 2009
- Source
- Proceedings of the National Academy of Sciences of the United States of America 106(35): 14896-14901 (Journal)
- Registered Authors
- Anderson, Ryan, Hesselson, Daniel, Stainier, Didier
- Keywords
- zebrafish, pancreas, islet, insulin, lineage
- MeSH Terms
-
- Insulin-Secreting Cells/cytology*
- Insulin-Secreting Cells/metabolism*
- Cell Differentiation
- Insulin/metabolism
- Genes, Reporter*
- Interphase*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Molecular Probe Techniques
- Animals
- Gene Expression Regulation, Developmental
- Integrases/analysis*
- Integrases/genetics
- Integrases/metabolism
- Cell Proliferation*
- PubMed
- 19706417 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Hesselson, D., Anderson, R.M., Beinat, M., and Stainier, D.Y. (2009) Distinct populations of quiescent and proliferative pancreatic β-cells identified by HOTcre mediated labeling. Proceedings of the National Academy of Sciences of the United States of America. 106(35):14896-14901.
Abstract
Pancreatic beta-cells are critical regulators of glucose homeostasis, and they vary dramatically in their glucose stimulated metabolic response and levels of insulin secretion. It is unclear whether these parameters are influenced by the developmental origin of individual beta-cells. Using HOTcre, a Cre-based genetic switch that uses heat-induction to precisely control the temporal expression of transgenes, we labeled two populations of beta-cells within the developing zebrafish pancreas. These populations originate in distinct pancreatic buds and exhibit gene expression profiles suggesting distinct functions during development. We find that the dorsal bud derived beta-cells are quiescent and exhibit a marked decrease in insulin expression postembryonically. In contrast, ventral bud derived beta-cells proliferate actively, and maintain high levels of insulin expression compared with dorsal bud derived beta-cells. Therapeutic strategies to regulate beta-cell proliferation and function are required to cure pathological states that result from excessive beta-cell proliferation (e.g., insulinoma) or insufficient beta-cell mass (e.g., diabetes mellitus). Our data reveal the existence of distinct populations of beta-cells in vivo and should help develop better strategies to regulate beta-cell differentiation and proliferation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping