PUBLICATION
Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms
- Authors
- Ries, J., Yu, S.R., Burkhardt, M., Brand, M., and Schwille, P.
- ID
- ZDB-PUB-090807-8
- Date
- 2009
- Source
- Nature Methods 6(9): 643-645 (Journal)
- Registered Authors
- Brand, Michael, Yu, Rachel Shuizi
- Keywords
- none
- MeSH Terms
-
- Animals
- Fibroblast Growth Factors/metabolism*
- Protein Binding
- Receptor, Fibroblast Growth Factor, Type 1/metabolism*
- Receptor, Fibroblast Growth Factor, Type 4/metabolism*
- Receptors, Fibroblast Growth Factor/metabolism*
- Spectrometry, Fluorescence/methods*
- Zebrafish/embryology
- Zebrafish Proteins/metabolism*
- PubMed
- 19648917 Full text @ Nat. Methods
Citation
Ries, J., Yu, S.R., Burkhardt, M., Brand, M., and Schwille, P. (2009) Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms. Nature Methods. 6(9):643-645.
Abstract
Analysis of receptor-ligand interactions in vivo is key to biology but poses a considerable challenge to quantitative microscopy. Here we combine static-volume, two-focus and dual-color scanning fluorescence correlation spectroscopy to solve this task at cellular resolution in complex biological environments. We quantified the mobility of fibroblast growth factor receptors Fgfr1 and Fgfr4 in cell membranes of living zebrafish embryos and determined their in vivo binding affinities to their ligand Fgf8.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping