|ZFIN ID: ZDB-PUB-090622-11|
Mosaic hoxb4a neuronal pleiotropism in zebrafish caudal hindbrain
Ma, L.H., Punnamoottil, B., Rinkwitz, S., and Baker, R.
|Source:||PLoS One 4(6): e5944 (Journal)|
|Registered Authors:||Baker, Robert, Ma, Leung-Hang Omicron, Punnamoottil, Beena, Rinkwitz, Silke|
|Keywords:||Neurons, Hindbrain, Yellow fluorescent protein, Neural pathways, Zebrafish, Cerebellum, Spinal cord, Mutant genotypes|
|PubMed:||19536294 Full text @ PLoS One|
Ma, L.H., Punnamoottil, B., Rinkwitz, S., and Baker, R. (2009) Mosaic hoxb4a neuronal pleiotropism in zebrafish caudal hindbrain. PLoS One. 4(6):e5944.
ABSTRACTTo better understand how individual genes and experience influence behavior, the role of a single homeotic unit, hoxb4a, was comprehensively analyzed in vivo by clonal and retrograde fluorescent labeling of caudal hindbrain neurons in a zebrafish enhancer-trap YFP line. A quantitative spatiotemporal neuronal atlas showed hoxb4a activity to be highly variable and mosaic in rhombomere 7-8 reticular, motoneuronal and precerebellar nuclei with expression decreasing differentially in all subgroups through juvenile stages. The extensive Hox mosaicism and widespread pleiotropism demonstrate that the same transcriptional protein plays a role in the development of circuits that drive behaviors from autonomic through motor function including cerebellar regulation. We propose that the continuous presence of hoxb4a positive neurons may provide a developmental plasticity for behavior-specific circuits to accommodate experience- and growth-related changes. Hence, the ubiquitous hoxb4a pleitropism and modularity likely offer an adaptable transcriptional element for circuit modification during both growth and evolution.