ZFIN ID: ZDB-PUB-090616-54
Regulation of slow and fast muscle myofibrillogenesis by Wnt/beta-catenin and myostatin signaling
Tee, J.M., van Rooijen, C., Boonen, R., and Zivkovic, D.
Date: 2009
Source: PLoS One   4(6): e5880 (Journal)
Registered Authors: van Rooijen, Carina
Keywords: Embryos, Muscle fibers, Muscle differentiation, Somites, Fast-twitch muscle fibers, Slow-twitch muscle fibers, Wnt signaling cascade, Zebrafish
MeSH Terms:
  • Animals
  • Cell Proliferation
  • Cyclin-Dependent Kinase Inhibitor p21/metabolism
  • Epistasis, Genetic
  • Gene Expression Regulation*
  • Gene Expression Regulation, Developmental*
  • Hypertrophy
  • Muscle Fibers, Fast-Twitch/pathology*
  • Muscle Fibers, Slow-Twitch/pathology*
  • Muscles/embryology*
  • Myostatin/metabolism*
  • Signal Transduction
  • Wnt Proteins/metabolism*
  • Zebrafish/embryology*
  • beta Catenin/metabolism*
PubMed: 19517013 Full text @ PLoS One
Deviation from proper muscle development or homeostasis results in various myopathic conditions. Employing genetic as well as chemical intervention, we provide evidence that a tight regulation of Wnt/beta-catenin signaling is essential for muscle fiber growth and maintenance. In zebrafish embryos, gain-of-Wnt/beta-catenin function results in unscheduled muscle progenitor proliferation, leading to slow and fast muscle hypertrophy accompanied by fast muscle degeneration. The effects of Wnt/beta-catenin signaling on fast muscle hypertrophy were rescued by misexpression of Myostatin or p21(CIP/WAF), establishing an in vivo regulation of myofibrillogenesis by Wnt/beta-catenin signaling and Myostatin. Epistatic analyses suggest a possible genetic interaction between Wnt/beta-catenin and Myostatin in regulation of slow and fast twitch muscle myofibrillogenesis.