PUBLICATION
Wastewater treatment effluent alters nucleotide excision repair in zebrafish (Danio rerio)
- Authors
- Notch, E.G., and Mayer, G.D.
- ID
- ZDB-PUB-090601-2
- Date
- 2009
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 150(2): 307-313 (Journal)
- Registered Authors
- Keywords
- Wastewater, Zebrafish, Nucleotide excision repair, Vitellogenin, CYP1A1
- MeSH Terms
-
- Animals
- Biomarkers/metabolism
- Cells, Cultured
- Cytochrome P-450 CYP1A1/genetics
- DNA Adducts/metabolism
- DNA Repair/drug effects*
- DNA-Binding Proteins/genetics
- Endocrine Disruptors/adverse effects
- Estrogens/adverse effects
- Fish Proteins/genetics
- Gene Expression Regulation/drug effects
- Humans
- Liver/drug effects*
- Liver/metabolism
- Maine
- Male
- Polycyclic Aromatic Hydrocarbons/adverse effects
- Promoter Regions, Genetic/drug effects
- RNA, Messenger/metabolism
- Receptors, Aryl Hydrocarbon/agonists
- Receptors, Estrogen/agonists
- Seasons
- Sewage/adverse effects*
- Transfection
- Vitellogenins/genetics
- Water Pollutants, Chemical/adverse effects*
- Water Purification*
- Xeroderma Pigmentosum Group A Protein/genetics
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- PubMed
- 19467346 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Notch, E.G., and Mayer, G.D. (2009) Wastewater treatment effluent alters nucleotide excision repair in zebrafish (Danio rerio). Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 150(2):307-313.
Abstract
Wastewater treatment effluent is a complex mixture of anthropogenic pollutants including estrogenic substances and chemicals, such as polyaromatic hydrocarbons, that cause bulky DNA adducts. Significant research focuses on reproductive effects of aquatic estrogens from wastewater treatment plants. However, other studies suggest genotoxic and immunological effects occur at lower concentrations of wastewater treatment effluent than reproductive endpoints. Recently, effects of estrogen on DNA repair processes in zebrafish have been suggested as a possible mechanism by which estrogen can modulate incidence of DNA mutations. Because wastewater treatment facilities are a significant source of estrogenic compounds, we hypothesized that exposure to whole effluents would also affect DNA repair in zebrafish (Danio rerio). Exposure to effluent from multiple treatment facilities in northern Maine decreased repair of DNA adducts in zebrafish liver cells. Expression of two nucleotide excision repair genes, XPC and XPA, were quantified and showed varied response after exposure in adult male zebrafish. Evidence of estrogen and aryl hydrocarbon receptor activation after exposure varied between treatment plants and temporally within treatment plants when evaluated using a traditional biomarkers, vitellogenin-1 (VTG) and, cytochrome p450 1A1 (CYP1A1) mRNA abundance. This research highlights continuing importance of examining non-reproductive effects of wastewater treatment effluent.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping