PUBLICATION
Effects on cell viability of three zebrafish testicular cell or tissue cryopreservation methods
- Authors
- Bono-Mestre, C., Cardona-Costa, J., and García-Ximenez, F.
- ID
- ZDB-PUB-090526-9
- Date
- 2009
- Source
- Cryo letters 30(2): 148-152 (Journal)
- Registered Authors
- Cardona-Costa, Jose
- Keywords
- cryopreservation, vitrification, testicular tissue, testicular cell, zebrafish
- MeSH Terms
-
- Animals
- Cell Survival/drug effects*
- Cryopreservation/methods*
- Cryoprotective Agents/pharmacology
- Male
- Testis/cytology*
- Testis/drug effects
- Zebrafish/growth & development*
- PubMed
- 19448864
Citation
Bono-Mestre, C., Cardona-Costa, J., and García-Ximenez, F. (2009) Effects on cell viability of three zebrafish testicular cell or tissue cryopreservation methods. Cryo letters. 30(2):148-152.
Abstract
In this work, three cryopreservation procedures were tested in order to obtain efficiently viable testicular cells after cryopreservation. Testicular cells of Wild type zebrafish males were frozen using an equilibrium protocol and testicular tissue fragments were cryopreserved with equilibrium freezing and vitrification procedures. Results showed that vitrification was significantly more efficient than freezing in terms of final cell survival (cell freezing: 14.4 percent, tissue freezing: 77.4 percent-85.5 percent, tissue vitrification: 94 percent). It must be noted that, in live cells, the presence of pseudopodia was frequently observed, which indicated their spermatogonial nature. Based on these results, the authors suggest that vitrification, with the subsequent elimination of connective tissue after warming, offers the best combination to rescue live testicular cells as a genetic conservation procedure in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping