PUBLICATION
Phylogenetic analysis of the insulin-like growth factor binding protein (IGFBP) and IGFBP-related protein gene families
- Authors
- Rodgers, B.D., Roalson, E.H., and Thompson, C.
- ID
- ZDB-PUB-090428-2
- Date
- 2008
- Source
- General and comparative endocrinology 155(1): 201-207 (Journal)
- Registered Authors
- Keywords
- Phylogenetics, IGFBP, IGFBP-rP
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Humans
- Insulin-Like Growth Factor Binding Protein 2/genetics
- Insulin-Like Growth Factor Binding Proteins/genetics*
- Molecular Sequence Data
- Multigene Family*
- Oncorhynchus mykiss/genetics
- Phylogeny*
- Sequence Homology, Amino Acid
- PubMed
- 17537438 Full text @ Gen. Comp. Endocrinol.
Citation
Rodgers, B.D., Roalson, E.H., and Thompson, C. (2008) Phylogenetic analysis of the insulin-like growth factor binding protein (IGFBP) and IGFBP-related protein gene families. General and comparative endocrinology. 155(1):201-207.
Abstract
Insulin-like growth factor (IGF) activity is regulated by six high affinity binding proteins (IGFBPs) and possibly by some of the nine IGFBP-related proteins (IGFBP-rPs). To determine the phylogenetic relationship of this proposed gene superfamily, we conducted maximum likelihood (ML) and Bayesian inference analyses on a matrix of amino acid sequences from a diversity of vertebrate species. A single most likely phylogram, ML bootstrap, and Bayesian consensus tree of 10,000,000 generations revealed a monophyletic IGFBP lineage independent of the IGFBP-rPs. The IGFBPs segregated into three distinct clades: IGFBP-1, -3, and -6. Subsequent gene duplication events within the IGFBP-1 and -3 clades resulted in the production and divergence of IGFBP-2 and -4 within the IGFBP-1 clade and IGFBP-5 in the IGFBP-3 clade. By contrast, the IGFBP-rPs were distributed paraphyletically into two clades: IGFBP-rP1, 5, and 6 in one clade and the CCN family (IGFBP-rP2-4,7-9) in another. A recently identified IGFBP-3 homolog in rainbow trout localized to the IGFBP-2 subclade. Subsequence analysis identified a RGD motif common to IGFBP-2 orthologs, but did not identify the nuclear localization sequence present in IGFBP-3 and -5 homologs. The putative trout IGFBP-3 was 36-55% identical to different IGFBP-2 proteins, but only 24-27% identical to IGFBP-3 proteins. These results suggest that the IGFBPs and IGFBP-rPs are at best distantly related and that the limited similarities likely resulted from exon shuffling. They also suggest that rainbow trout, and possibly other salmonids, possess two IGFBP-2 paralogs as the putative trout IGFBP-3 is misannotated.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping