PUBLICATION
Multiple embryo time-lapse imaging of zebrafish development
- Authors
- Herrgen, L., Schröter, C., Bajard, L., and Oates, A.C.
- ID
- ZDB-PUB-090424-8
- Date
- 2009
- Source
- Methods in molecular biology (Clifton, N.J.) 546: 243-254 (Chapter)
- Registered Authors
- Herrgen, Leah, Oates, Andrew
- Keywords
- Zebrafish, Embryogenesis, Brightfield imaging, Time-lapse, Morphogenetic movements, Population statistics, Quantification, Temperature control
- MeSH Terms
-
- Animals
- Developmental Biology/methods
- Embryo, Nonmammalian/anatomy & histology
- Embryonic Development*
- Image Processing, Computer-Assisted/methods*
- Temperature
- Time Factors
- Zebrafish/anatomy & histology
- Zebrafish/growth & development*
- PubMed
- 19378108 Full text @ Meth. Mol. Biol.
Citation
Herrgen, L., Schröter, C., Bajard, L., and Oates, A.C. (2009) Multiple embryo time-lapse imaging of zebrafish development. Methods in molecular biology (Clifton, N.J.). 546:243-254.
Abstract
Understanding the dynamics of developmental and cellular processes requires documentation of their changes with appropriate temporal and spatial resolution. Furthermore, simultaneous recording from a population of embryos under identical conditions allows statistical estimates of precision and variability to be made. This chapter describes a protocol for time-lapse microscopy of multiple embryos in parallel developing under tightly controlled conditions. This method is currently best suited to follow tissue-scale morphogenetic movements with temporal resolution in the minute range, for hours or even days. Applications of the method include the comparison of the dynamics of a process of interest between groups of wild-type embryos and their mutant siblings or between embryos treated with different chemical compounds. Temperature control allows for the investigation of the temperature dependence of a process of interest.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping