PUBLICATION

Expression regulation of Na+-K+-ATPase {alpha}1-subunit subtypes in zebrafish gill ionocytes

Authors
Liao, B.K., Chen, R.D., and Hwang, P.P.
ID
ZDB-PUB-090424-30
Date
2009
Source
American journal of physiology. Regulatory, integrative and comparative physiology   296(6): R1897-R1906 (Journal)
Registered Authors
Hwang, Pung Pung
Keywords
none
MeSH Terms
  • Animals
  • Calcium/metabolism
  • Calcium Channels/metabolism
  • Chlorides/metabolism
  • Gene Expression Regulation, Enzymologic
  • Gills/cytology
  • Gills/enzymology*
  • Phylogeny
  • Protein Subunits
  • Proton-Translocating ATPases/metabolism
  • RNA, Messenger/metabolism
  • Sodium/metabolism
  • Sodium Chloride Symporters/metabolism
  • Sodium-Hydrogen Exchangers/metabolism
  • Sodium-Potassium-Exchanging ATPase/genetics
  • Sodium-Potassium-Exchanging ATPase/metabolism*
  • Water-Electrolyte Balance
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
19386990 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
Abstract
In zebrafish (Danio rerio), 6 distinct Na-K-ATPase (NKA) alpha1 subunit genes have been identified, and 4 of them, zATP1a1a.1, zATP1a1a.2, zATP1a1a.4, and zATP1a1a.5, are expressed in embryonic skin, where different types of ionocytes appear. The present study attempted to test a hypothesis of whether these NKA alpha1 paralogues are specifically expressed and function in respective ionocytes. Double fluorescence in situ hybridization analysis demonstrated the specific expression of zATP1a1a.1, zATP1a1a.2, and zATP1a1a.5 in NKA-rich (NaR) cells, Na(+)-Cl(-) cotransporter (NCC)-expressing cells, and H(+)-ATPase-rich (HR) cells, respectively, based the colocalization of the 3 NKA alpha1 genes with marker genes of the respective ionocytes (epithelial Ca(2+) channel in NaR cells; NCC in NCC cells; and H(+)-ATPase and Na(+)/H(+) exchanger 3b in HR cells). The mRNA expression (by real-time PCR) of zATP1a1a.1, zATP1a1a.2, and zATP1a1a.5 were respectively upregulated by low-Ca(2+), low-Cl(-), and low-Na(+) fresh water, which had previously been reported to stimulate uptake functions of Ca(2+), Cl(-), and Na(+). However, zATP1a1a.4 was not colocalized with any of the 3 types of ionocytes, nor did its mRNA respond to the ambient ions examined. Taken together, zATP1a1a.1, zATP1a1a.2, and zATP1a1a.5 may provide driving force for Na(+) coupled cotransporter activity specifically in NaR, NCC, and HR cells, respectively. Key words: mitochondrion-rich cells, ion regulation, osmoregulation, fish.
Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping