PUBLICATION
Nitroreductase-mediated cell ablation in transgenic zebrafish embryos
- Authors
- Pisharath, H., and Parsons, M.J.
- ID
- ZDB-PUB-090424-2
- Date
- 2009
- Source
- Methods in molecular biology (Clifton, N.J.) 546: 133-143 (Chapter)
- Registered Authors
- Parsons, Michael
- Keywords
- Ablation, Nitroreductase, Metronidazole, Prodrug, Transactivation, Gal4, UAS
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/embryology
- Animals, Genetically Modified/genetics
- Animals, Genetically Modified/metabolism*
- Anti-Infective Agents/pharmacology
- Apoptosis/drug effects*
- DNA/genetics
- DNA/metabolism
- Escherichia coli Proteins/administration & dosage*
- Escherichia coli Proteins/biosynthesis
- Genetic Engineering/methods
- Green Fluorescent Proteins
- Metronidazole/pharmacology
- Microinjections
- Microscopy, Confocal
- Nitroreductases/administration & dosage*
- Nitroreductases/biosynthesis
- Prodrugs/pharmacology
- Recombinant Fusion Proteins/administration & dosage
- Recombinant Fusion Proteins/biosynthesis
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism*
- PubMed
- 19378102 Full text @ Meth. Mol. Biol.
Citation
Pisharath, H., and Parsons, M.J. (2009) Nitroreductase-mediated cell ablation in transgenic zebrafish embryos. Methods in molecular biology (Clifton, N.J.). 546:133-143.
Abstract
Prodrug dependent cell ablation is a method that allows inducible and spatially restricted cell destruction. We describe transgenic methods to express the Escherichia coli nfsB in a tissue restricted manner in the zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme that can render prodrugs such as metronidazole (Met) cytotoxic. Using the expression of NTR fused to a fluorescent protein, one can simultaneously make cells susceptible to prodrug treatment and visualize cell ablation as it occurs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping