|ZFIN ID: ZDB-PUB-090424-2|
Nitroreductase-mediated cell ablation in transgenic zebrafish embryos
Pisharath, H., and Parsons, M.J.
|Source:||Methods in molecular biology (Clifton, N.J.) 546: 133-143 (Chapter)|
|Registered Authors:||Parsons, Michael|
|Keywords:||Ablation, Nitroreductase, Metronidazole, Prodrug, Transactivation, Gal4, UAS|
|PubMed:||19378102 Full text @ Meth. Mol. Biol.|
Pisharath, H., and Parsons, M.J. (2009) Nitroreductase-mediated cell ablation in transgenic zebrafish embryos. Methods in molecular biology (Clifton, N.J.). 546:133-143.
ABSTRACTProdrug dependent cell ablation is a method that allows inducible and spatially restricted cell destruction. We describe transgenic methods to express the Escherichia coli nfsB in a tissue restricted manner in the zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme that can render prodrugs such as metronidazole (Met) cytotoxic. Using the expression of NTR fused to a fluorescent protein, one can simultaneously make cells susceptible to prodrug treatment and visualize cell ablation as it occurs.
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