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ZFIN ID: ZDB-PUB-090424-10
Imaging zebrafish embryos by two-photon excitation time-lapse microscopy
Carvalho, L., and Heisenberg, C.P.
Date: 2009
Source: Methods in molecular biology (Clifton, N.J.) 546: 273-287 (Chapter)
Registered Authors: Heisenberg, Carl-Philipp
Keywords: Zebrafish, Two-photon excitation microscopy, Time-lapse, Cell migration, YSL nuclei, Gastrulation
MeSH Terms:
  • Animals
  • Cell Movement
  • Gastrulation
  • Genes, Reporter
  • Green Fluorescent Proteins
  • Image Processing, Computer-Assisted/methods
  • Microscopy, Confocal/instrumentation
  • Microscopy, Confocal/methods*
  • Morphogenesis
  • Photons
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Recombinant Fusion Proteins/biosynthesis
  • Recombinant Fusion Proteins/genetics
  • Time Factors
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed: 19378110 Full text @ Meth. Mol. Biol.
The zebrafish is a favorite model organism to study tissue morphogenesis during development at a subcellular level. This largely results from the fact that zebrafish embryos are transparent and thus accessible to various imaging techniques, such as confocal and two-photon excitation (2PE) microscopy. In particular, 2PE microscopy has been shown to be useful for imaging deep cell layers within the embryo and following tissue morphogenesis over long periods. This chapter describes how to use 2PE microscopy to study morphogenetic movements during early zebrafish embryonic development, providing a general blueprint for its use in zebrafish.