Anatomy of zebrafish cerebellum and screen for mutations affecting its development

Bae, Y.K., Kani, S., Shimizu, T., Tanabe, K., Nojima, H., Kimura, Y., Higashijima, S.I., and Hibi, M.
Developmental Biology   330(2): 406-426 (Journal)
Registered Authors
Bae, Young Ki, Hibi, Masahiko, Higashijima, Shin-ichi, Kani, Shuichi, Nojima, Hideaki, Shimizu, Takashi, Tanabe, Koji
Cerebellum, Hindbrain, Purkinje cells, Granule cells, Eurydendroid cells, Climbing fibers, Mossy fibers, Parallel fibers, Neural circuits, Mutant screening, Zebrafish
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Cerebellum/anatomy & histology*
  • Cerebellum/embryology
  • Cerebellum/metabolism
  • DNA Primers
  • Glutamates/metabolism
  • Immunohistochemistry
  • In Situ Hybridization
  • Molecular Sequence Data
  • Mutation*
  • Neuroglia/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • gamma-Aminobutyric Acid/metabolism
19371731 Full text @ Dev. Biol.
The cerebellum is important for the integration of sensory perception and motor control, but its structure has mostly been studied in mammals. Here, we describe the cell types and neural tracts of the adult zebrafish cerebellum using molecular markers and transgenic lines. Cerebellar neurons are categorized to two major groups: GABAergic and glutamatergic neurons. The Purkinje cells, which are GABAergic neurons, express parvalbumin7, carbonic anhydrase8, and aldolase C like (zebrin II). The glutamatergic neurons are vglut1(+) granule cells and vglut2(high) cells, which receive Purkinje cell inputs; some vglut2(high) cells are eurydendroid cells, which are equivalent to the mammalian deep cerebellar nuclei. We found olig2(+) neurons in the adult cerebellum and ascertained that at least some of them are eurydendroid cells. We identified markers for climbing and mossy afferent fibers, efferent fibers, and parallel fibers from granule cells. Furthermore, we found that the cerebellum-like structures in the optic tectum and antero-dorsal hindbrain show similar Parvalbmin7 and Vglut1 expression profiles as the cerebellum. The differentiation of GABAergic and glutamatergic neurons begins 3 days post fertilization (dpf), and layers are first detectable 5 dpf. Using anti-Parvalbumin7 and Vglut1 antibodies to label Purkinje cells and granule cell axons, respectively, we screened for mutations affecting cerebellar neuronal development and the formation of neural tracts. Our data provide a platform for future studies of zebrafish cerebellar development.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes