PUBLICATION

Bacterial artificial chromosome transgenesis for zebrafish

Authors
Yang, Z., Jiang, H., and Lin, S.
ID
ZDB-PUB-090422-27
Date
2009
Source
Methods in molecular biology (Clifton, N.J.)   546: 103-116 (Chapter)
Registered Authors
Jiang, Hong, Lin, Shuo, Yang, Zhongan
Keywords
Transgenic zebrafish, Bacterial artificial chromosomes, BAC modification, Fluorescent reporter genes, GFP/RFP
MeSH Terms
  • Animals
  • Chromosomes, Artificial, Bacterial/metabolism*
  • DNA/genetics
  • DNA/metabolism
  • Databases, Nucleic Acid
  • Escherichia coli/genetics
  • Escherichia coli/metabolism
  • Gene Transfer Techniques*
  • Genes, Reporter
  • Genetic Engineering/methods
  • Green Fluorescent Proteins
  • Luminescent Proteins
  • Recombination, Genetic
  • Sequence Analysis, DNA
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed
19378100 Full text @ Meth. Mol. Biol.
Abstract
Transgenesis using bacterial artificial chromosomes (BAC) allows greater fidelity in directing desirable expression of transgenes. Application of this technology in the optically transparent zebrafish with fluorescent protein reporters enables unparalleled visual analysis of gene expression in a living organism. We have developed a streamlined procedure of directly selecting multiple BAC clones based on public sequence databases followed by rapid modification with green fluorescent protein or red fluorescent protein for transgenic analysis in zebrafish. In this chapter, experimental procedures for BAC DNA preparation and generation of transgenic zebrafish lines by microinjection are described.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping