PUBLICATION

Effects of Adrenergic Agents on the Expression of Zebrafish (Danio rerio) Vitellogenin Ao1

Authors
Yin, N., Jin, X., He, J., and Yin, Z.
ID
ZDB-PUB-090422-11
Date
2009
Source
Toxicology and applied pharmacology   238(1): 20-26 (Journal)
Registered Authors
He, Jiangyan
Keywords
Zebrafish, vtgAo1, adrenergic agents, heart, liver
MeSH Terms
  • Adrenergic alpha-Agonists/pharmacology*
  • Adrenergic alpha-Antagonists/pharmacology
  • Adrenergic beta-Agonists/pharmacology*
  • Animals
  • Enzyme-Linked Immunosorbent Assay
  • Gene Expression Regulation/drug effects*
  • Isoproterenol/pharmacology
  • Liver/metabolism
  • Moxisylyte/pharmacology
  • Myocardium/metabolism
  • Phenylephrine/pharmacology
  • RNA, Messenger/drug effects
  • RNA, Messenger/metabolism
  • Receptors, Estrogen/antagonists & inhibitors
  • Tamoxifen/pharmacology
  • Vitellogenins/drug effects*
  • Vitellogenins/genetics
  • Zebrafish
PubMed
19371759 Full text @ Tox. App. Pharmacol.
Abstract
Teleost vitellogenins (VTGs) are large multidomain apolipoproteins, traditionally considered to be estrogen-responsive precursors of the major egg yolk proteins, expressed and synthesized mainly in hepatic tissue. The inducibility of VTGs has made them one of the most frequently used in vivo and in vitro biomarkers of exposure to estrogen-active substances. A significant level of zebrafish vtgAo1, a major estrogen responsive form, has been unexpectedly found in heart tissue in our present studies. Our studies on zebrafish cardiomyopathy, caused by adrenergic agonist treatment, suggest a similar protective function of the cardiac expressed vtgAo1. We hypothesize that its function is to unload surplus intracellular lipids in cardiomyocytes for "reverse triglyceride transportation" similar to that found in lipid transport proteins in mammals. Our results also demonstrated that zebrafish vtgAo1 mRNA expression in heart can be suppressed by both alpha-adrenergic agonist, phenylephrine (PE) and beta-adrenergic agonist, isoproterenol (ISO). Furthermore, the strong stimulation of zebrafish vtgAo1 expression in plasma induced by the beta-adrenergic antagonist, MOXIsylyl, was detected by Enzyme-Linked ImmunoSorbent Assay (ELISA). Such stimulation cannot be suppressed by taMOXIfen, an antagonist to estrogen receptors. Thus, our present data indicate that the production of teleost VTG in vivo can be regulated not only by estrogenic agents, but by adrenergic signals as well.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes