ZFIN ID: ZDB-PUB-090407-2
Molecular cloning and expression of phospholipase C epsilon 1 in zebrafish
Zhou, W., and Hildebrandt, F.
Date: 2009
Source: Gene expression patterns : GEP   9(5): 282-288 (Journal)
Registered Authors: Zhou, Weibin
Keywords: Zebrafish, Phospholipase, PLCE1, Rhombomere, Horizontal myoseptum, Muscle pioneer cells, Pronephros, Glomerulus, Nephrotic syndrome
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • DNA, Complementary/chemistry
  • DNA, Complementary/genetics
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Female
  • Gene Expression Profiling*
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Immunohistochemistry
  • In Situ Hybridization
  • Male
  • Molecular Sequence Data
  • Phosphoinositide Phospholipase C/genetics*
  • Phosphoinositide Phospholipase C/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 19332147 Full text @ Gene Expr. Patterns
Phospholipase C (PLC) is a key enzyme that generates inositol 1, 4, 5 - triphosphate (IP3) and diacylglycerol (DAG), two second messengers in signal transduction. Phospholipase Cepsilon (PLCE1) is a unique member of the PLC family in that it carries both RasGEF and Ras-associating (RA) domains and may serve as an activator and an effector of small GTPases. Recently, mutations in PLCE1 have been associated with early-onset nephrotic syndrome. We have identified the zebrafish ortholog of PLCE1 and characterized its expression pattern in zebrafish embryos by in situ hybridization. Zebrafish plce1 gene encodes a protein of 2, 248 amino acids that shares 56% identity with mammalian PLCepsilon. During zebrafish embryonic development, plce1 is expressed in a dynamic pattern in a variety of organs, such as brain, muscle, liver, and pronephric glomeruli. Our results suggest that zebrafish may be used as a model organism to address the function of PLCepsilon during the development of these organs.