PUBLICATION
Molecular cloning and expression of phospholipase C epsilon 1 in zebrafish
- Authors
- Zhou, W., and Hildebrandt, F.
- ID
- ZDB-PUB-090407-2
- Date
- 2009
- Source
- Gene expression patterns : GEP 9(5): 282-288 (Journal)
- Registered Authors
- Hildebrandt, Friedhelm, Zhou, Weibin
- Keywords
- Zebrafish, Phospholipase, PLCE1, Rhombomere, Horizontal myoseptum, Muscle pioneer cells, Pronephros, Glomerulus, Nephrotic syndrome
- MeSH Terms
-
- Gene Expression Regulation, Enzymologic
- In Situ Hybridization
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/embryology
- Zebrafish/genetics*
- Sequence Analysis, DNA
- DNA, Complementary/chemistry
- DNA, Complementary/genetics
- Animals
- Sequence Homology, Amino Acid
- Molecular Sequence Data
- Cloning, Molecular
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Amino Acid Sequence
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Phosphoinositide Phospholipase C/genetics*
- Phosphoinositide Phospholipase C/metabolism
- Female
- Immunohistochemistry
- Gene Expression Profiling*
- Male
- PubMed
- 19332147 Full text @ Gene Expr. Patterns
Citation
Zhou, W., and Hildebrandt, F. (2009) Molecular cloning and expression of phospholipase C epsilon 1 in zebrafish. Gene expression patterns : GEP. 9(5):282-288.
Abstract
Phospholipase C (PLC) is a key enzyme that generates inositol 1, 4, 5 - triphosphate (IP3) and diacylglycerol (DAG), two second messengers in signal transduction. Phospholipase Cepsilon (PLCE1) is a unique member of the PLC family in that it carries both RasGEF and Ras-associating (RA) domains and may serve as an activator and an effector of small GTPases. Recently, mutations in PLCE1 have been associated with early-onset nephrotic syndrome. We have identified the zebrafish ortholog of PLCE1 and characterized its expression pattern in zebrafish embryos by in situ hybridization. Zebrafish plce1 gene encodes a protein of 2, 248 amino acids that shares 56% identity with mammalian PLCepsilon. During zebrafish embryonic development, plce1 is expressed in a dynamic pattern in a variety of organs, such as brain, muscle, liver, and pronephric glomeruli. Our results suggest that zebrafish may be used as a model organism to address the function of PLCepsilon during the development of these organs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping