ZFIN ID: ZDB-PUB-090324-8
Transcriptional control of Rohon-Beard sensory neuron development at the neural plate border
Rossi, C.C., Kaji, T., and Artinger, K.B.
Date: 2009
Source: Developmental dynamics : an official publication of the American Association of Anatomists   238(4): 931-943 (Journal)
Registered Authors: Artinger, Kristin Bruk, Kaji, Takao
Keywords: Rohon-Beard sensory neurons, prdm1a, dlx3b, dlx4b, neurogenin1
MeSH Terms:
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Gastrulation
  • Gene Expression Regulation, Developmental/genetics
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Neural Plate/cytology
  • Neural Plate/embryology*
  • Neural Plate/metabolism*
  • Neurogenesis
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism
  • SOXB1 Transcription Factors/genetics
  • SOXB1 Transcription Factors/metabolism
  • Sensory Receptor Cells/cytology*
  • Sensory Receptor Cells/metabolism*
  • Transcription, Genetic/genetics*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 19301392 Full text @ Dev. Dyn.
Rohon-Beard (RB) mechanosensory neurons are among the first sensory neurons to develop, and the process by which they adopt their fate is not completely understood. RBs form at the neural plate border (NPB), the junction between neural and epidermal ectoderm, and require the transcription factor prdm1a. Here, we show that prior to RB differentiation, prdm1a overlaps extensively with the epidermal marker dlx3b but shows little overlap with the neuroectodermal markers sox3 and sox19a. Birthdating analysis reveals that the majority of RBs are born during gastrulation in zebrafish, suggesting that it is during this period that RBs become specified. Expression analysis in prdm1a and neurogenin1 mutant and dlx3b/dlx4b morpholino-injected embryos suggests that prdm1a is upstream of dlx3b, dlx4b, and neurogenin1 at the NPB. mRNA for neurogenin1 or dlx3b/dlx4b can rescue the lack of RBs in prdm1a mutants. Based on these data, we suggest a preliminary gene regulatory network for RB development.