ZFIN ID: ZDB-PUB-090318-10
Lin9 is required for mitosis and cell survival during early zebrafish development
Kleinschmidt, M.A., Wagner, T.U., Liedtke, D., Spahr, S., Samans, B., and Gaubatz, S.
Date: 2009
Source: The Journal of biological chemistry   284(19): 13119-13127 (Journal)
Registered Authors: Liedtke, Daniel, Wagner, Toni
Keywords: none
MeSH Terms:
  • Animals
  • Apoptosis
  • Cell Cycle Proteins/genetics*
  • Cell Cycle Proteins/metabolism*
  • Cell Survival/physiology
  • Embryo, Nonmammalian/physiology*
  • Embryonic Development/physiology*
  • Flow Cytometry
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental*
  • In Situ Hybridization
  • Mitosis/physiology*
  • Morpholines/pharmacology
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
PubMed: 19278998 Full text @ J. Biol. Chem.
LIN9 has been described as a regulator of G1/S and G2/M progression of the cell cycle in invertebrates and human cell lines. To elucidate the in vivo function of LIN9 during vertebrate development, we took advantage of the teleost zebrafish (Danio rerio). By means of antisense morpholinos we show here that Lin9-depleted embryonic cells accumulate in mitosis. Flow cytometry and confocal microscopy data demonstrate that the delay in mitotic progression is followed by apoptosis, which strongly manifests in the developing CNS. In accordance with these findings we identified a cohort of Lin9 regulated genes required for different mitotic processes, including mitotic entry, metaphase/anaphase transition and cytokinesis. Our data establish LIN9 as an essential regulator of mitosis in vertebrate development.